Epidemiological study of p53 tumor suppressor gene mutations in patients from Luxembourg and the German Saar region with an advanced colorectal cancer using PCR-SSCP analysis

Pauly M., Schmitz M., Kayser I., Lagoda Pierre, Tuereci O., Kerschen A., Weber Jacques, Seitz G., Hentges F., Dicato M.. 1998. Epidemiological study of p53 tumor suppressor gene mutations in patients from Luxembourg and the German Saar region with an advanced colorectal cancer using PCR-SSCP analysis. Biomedicine and Pharmacotherapy, 52 (5) : 220-228.

Autre titre : Etude épidémiologique, à l'aide d'une analyse PCR-SSCP, du gene p53, supresseur de tumeurs, chez des patients luxembougeois ou habitant la Sahr allemande qui présentent un cancer colorectal avancé

Résumé : Mutations in the p53 tumor suppressor gene are usually associated with an advanced development of colorectal cancer characterized by the transition from the adenoma to the carcinoma stage. We used the polymerase chain reaction (PCR) followed by single-strand conformation polymorphism (SSCP) analysis to screen for the presence of mutations in the p53 gene of patients from Luxembourg and the German Saar region with colorectal cancers at various developmental stages. While we detected no mutations in 16 colic polypi at an early to intermediate stage (adenoma), we revealed seven (13.7C%c) non-silent point mutations (transitions) in exons 5 to 9 of the p53 gene in 51 colorectal tumors at a late stage (carcinoma). In addition to confirming previous observations, these results show that PCR-SSCP analysis can provide both a sensitive and rapid method for the genetic determination of the histopathological stage of colorectal samples.

Mots-clés Agrovoc : gène, néoplasme, technique analytique, PCR, diagnostic

Classification Agris : U30 - Méthodes de recherche

Auteurs et affiliations

• Pauly M.
• Schmitz M.
• Kayser I.
• Lagoda Pierre, CIRAD-AMIS-BIOTROP (FRA)
• Tuereci O.
• Kerschen A.
• Weber Jacques, CIRAD-DG-DS (FRA)
• Seitz G.
• Hentges F.
• Dicato M.

Source : Cirad - Agritrop (https://agritrop.cirad.fr/401110/)

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