Maillard Jean-Charles, Renard C., Chardon P., Chantal Isabelle, Bensaïd Albert. 1999. Characterization of 18 new BoLA-DRB3 alleles. Animal Genetics, 30 : 200-203.
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Autre titre : Caractérisation de 18 nouveaux alléles BoLA-DRB3
Résumé : The second exon of the bovine MHC class II DRB3 gene was amplified by polymerase chain reaction (PCR) from DNA samples of 568 zebu Brahman cattle (Bos indicus) from Martinique (French West Indies). Cloning of these PCR products allowed the isolation of both alleles from each animal, which were characterized by the PCR-restriction fragment length polymorphism (RFLP) technique using the restriction enzymes Rsal, BstYl and Haelll. Four new PCR-RFLP patterns were obtained by digestion with Rsal. These patterns were named 'v', w,'x' and 'y' continuing the accepted nomenclature. Sequencing of each allele allowed the identification of 18 new BoLA-DRB3 exon 2 nucleotide sequences and their deduced amino acid sequences.
Mots-clés Agrovoc : bovin, zébu, séquence nucléotidique, polymorphisme génétique, PCR, RFLP
Mots-clés géographiques Agrovoc : Martinique, France
Classification Agris : L10 - Génétique et amélioration des animaux
Auteurs et affiliations
- Maillard Jean-Charles, CIRAD-EMVT-SANTE ANIMALE (FRA)
- Renard C.
- Chardon P.
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Chantal Isabelle, CIRAD-EMVT-SANTE ANIMALE (FRA)
ORCID: 0000-0002-3429-484X
- Bensaïd Albert, CIRAD-EMVT-SANTE ANIMALE (GLP)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/401324/)
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