Strategy to study functionality of putative promotor from Hevea brasiliensis. Attemps of Agrobacterium tumefaciens-mediated gene transfer in various tissue

Montoro Pascal, Pujade-Renaud Valérie, Teinseree Nongluk. 1997. Strategy to study functionality of putative promotor from Hevea brasiliensis. Attemps of Agrobacterium tumefaciens-mediated gene transfer in various tissue. In : Seminar on the biochemical and molecular tools for exploitation diagnostic and rubber tree improvement. Workshop on electrophoresis application to rubber tree clone identification. Mahidol University, RRIT, ORSTOM, CIRAD. Bangkok : Mahidol University, 6 p. Seminar on the Biochemical and Molecular Tools for Exploitation Diagnostic and Rubber Tree Improvement, Bangkok, Thaïlande, 20 Octobre 1997/22 Octobre 1997.

Autre titre : Stratégie d'étude du fonctionnement du promoteur putatif d'Hevea brasiliensis. Tentatives de transfert de gène par Agrobacterium tumefaciens dans divers tissus

Résumé : Genetic transformation via #Agrobacterium tumefaciens# was carried out in various plant organs and #in vitro# cell tissue of #Hevea brasiliensis#. Transient expression of the gusA reporter gene was recorded in both tegument calli and longterm friable calli. Other differentiated organs and embryos are not susceptible to the infection with 5 different agrobacteria strains. Northern blot analysis showed that the ethylene-inducible glutamine synthetase GS1 is expressed in friable calli as in latex cells of ethylene-stimulated trees. Therefore, such tissue might be used for the functional analysis of GS1 promoter. On the contrary, Hevein expression is strictly latex cell-specific and we assume that its promotor can be studied only in differentiated latex cell from transgenic rubber tree.

Mots-clés Agrovoc : Hevea brasiliensis, Agrobacterium tumefaciens, transfert de gène, transformation génétique, génie génétique

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Montoro Pascal, CIRAD-CP-HEVEA (THA)
• Pujade-Renaud Valérie, CIRAD-CP-HEVEA (THA)
• Teinseree Nongluk

Source : Cirad - Agritrop (https://agritrop.cirad.fr/464993/)

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