Mapping of avirulence genes in the rice blast fungus, Magnaporthe grisea, with RFLP and RAPD markers

Dioh Waly, Tharreau Didier, Nottéghem Jean-Loup, Orbach M., Lebrun Marc-Henri. 2000. Mapping of avirulence genes in the rice blast fungus, Magnaporthe grisea, with RFLP and RAPD markers. Molecular Plant-Microbe Interactions, 13 (2) : 217-227.

Autre titre : Cartographie de gènes de l'avirulence chez le champignon de la pyriculariose, Magnaporthe grisea, avec des marqueurs RFLP et RAPD

Résumé : Three genetically independent avirulence genes, AVR1-Irat7, AVR1-MedNoï, and AVR1-Ku86, were identified in a cross involving isolates Guy11 and 2/0/3 of the rice blast fungus, Magnaporthe grisea. Using 76 random progeny, we constructed a partial genetic map with restriction fragment length polymorphism (RFLP) markers revealed by probes such as the repeated sequences MGL/MGR583 and Pot3/MGR586, cosmids from the M. grisea genetic map, and a telomere sequence oligonucleotide. Avirulence genes AVR1-MedNoï and AVR1-Ku86 were closely linked to telomere RFLPs such as marker TelG (6 cM from AVR1-MedNoï) and TelF (4.5 cM from AVR1-Ku86). Avirulence gene AVR1-Irat7 was linked to a cosmid RFLP located on chromosome 1 and mapped at 20 cM from the avirulence gene AVR1-CO39. Using bulked segregant analysis, we identified 11 random amplified polymorphic DNA (RAPD) markers closely linked (0 to 10 cM) to the avirulence genes segregating in this cross. Most of these RAPD markers corresponded to junction fragments between known or new transposons and a single-copy sequence. Such junctions or the whole sequences of single-copy RAPD markers were frequently absent in one parental isolate. Singlecopy sequences from RAPD markers tightly linked to avirulence genes will be used for positional cloning.

Mots-clés Agrovoc : Magnaporthe grisea, maladie fongique, gène, pouvoir pathogène, carte génétique, RFLP, RAPD, marqueur génétique

Classification Agris : H20 - Maladies des plantes

Auteurs et affiliations

• Dioh Waly, CNRS (FRA)
• Tharreau Didier, CIRAD-CA-CALIM (FRA) ORCID: 0000-0003-3961-6120
• Nottéghem Jean-Loup, ENSAM [Ecole nationale supérieure agronomique de Montpellier] (FRA)
• Orbach M., University of Arizona (USA)
• Lebrun Marc-Henri, CNRS (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/475921/)

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