PLS1, a gene encoding a tetraspanin-like protein, is required for penetration of rice leaf by the fungal pathogen Magnaporthe grisea

Clergeot Pierre-Henri, Gourgues Mathieu, Cots Joaquim, Laurans F., Pepin R., Tharreau Didier, Nottéghem Jean-Loup, Lebrun Marc-Henri. 2001. PLS1, a gene encoding a tetraspanin-like protein, is required for penetration of rice leaf by the fungal pathogen Magnaporthe grisea. Proceedings of the National Academy of Sciences of the United States of America, 98 (12) : 6963-6968.

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Economie-gestion; Psychologie-éthologie-ergonomie

Résumé : We describe in this study punchless, a nonpathogenic mutant from the rice blast fungus M. grisea, obtained by plasmid-mediated insertional mutagenesis. As do most fungal plant pathogens, M. grisea differentiates an infection structure specialized for host penetration called the appressorium. We show that punchless differentiates appressoria that fail to breach either the leaf epidermis or artificial membranes such as cellophane. Cytological analysis of punchless appressoria shows that they have a cellular structure, turgor, and glycogen content similar to those of wild type before penetration, but that they are unable to differentiate penetration pegs. The inactivated gene, PLS1, encodes a putative integral membrane protein of 225 aa (PIs1p). A functional Plsl 1p-green fluorescent protein fusion protein was detected only in appressoria and was localized in plasma membranes and vacuoles. Pls1p is structurally related to the tetraspanin family. In animals, these proteins are components of membrane signaling complexes controlling cell differentiation, motility, and adhesion. We conclude that PLS1 controls an appressorial function essential for the penetration of the fungus into host leaves.

Mots-clés Agrovoc : Magnaporthe grisea, infection expérimentale, transmission des maladies, gène

Classification Agris : H20 - Maladies des plantes

Auteurs et affiliations

• Clergeot Pierre-Henri, CNRS (FRA)
• Gourgues Mathieu, CNRS (FRA)
• Cots Joaquim, CNRS (FRA)
• Laurans F., UCLB (FRA)
• Pepin R., UCLB (FRA)
• Tharreau Didier, CIRAD-CA-CALIM (FRA) ORCID: 0000-0003-3961-6120
• Nottéghem Jean-Loup, ENSAM [Ecole nationale supérieure agronomique de Montpellier] (FRA)
• Lebrun Marc-Henri, CNRS (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/479974/)

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