Vaysse Laurent, Dubreucq Eric, Pirat Jean-Luc, Galzy Pierre. 1997. Fatty hydroxamic acid biosynthesis in aqueous medium in the presence of the lipase-acyltransferase from Candida parapsilosis. Journal of Biotechnology, 53 (1) : 41-46.
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Résumé : The lipase-acyltransferase from Candida parapsilosis has been shown to catalyze fatty hydroxamic acid biosynthesis in a biphasic lipid/aqueous medium. The substrates of the reaction were an acyl donor (fatty acid or fatty acid methyl ester) and hydroxylamine. The transfer of acyl groups from a donor ester to hydroxylamine (aminolysis) was catalyzed preferentially to the reaction of free fatty acids. The highest synthesis activity was obtained in the presence of 1 M hydroxylamine at 45°C and pH 6. This work confirmed the originality of the enzyme from Candida parapsilosis, which acts more like an acyltransferase than an hydrolase. This feature makes it an enzyme of choice for the direct bioconversion of oils in aqueous medium.
Mots-clés Agrovoc : biosynthèse, acide hydroxamique, acide gras, acyltransférase, estérase, Candida parapsilosis, substrat de culture
Mots-clés complémentaires : Acide gras hydroxamique
Classification Agris : Q60 - Traitement des produits agricoles non alimentaires
Auteurs et affiliations
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Vaysse Laurent, ENSAM [Ecole nationale supérieure agronomique de Montpellier] (FRA)
ORCID: 0000-0003-3264-6116
- Dubreucq Eric, ENSAM [Ecole nationale supérieure agronomique de Montpellier] (FRA)
- Pirat Jean-Luc, ENSCM (FRA)
- Galzy Pierre, ENSAM [Ecole nationale supérieure agronomique de Montpellier] (FRA)
Autres liens de la publication
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