D'Hont Angélique, Le Cunff Loïc, Garsmeur Olivier, Paulet Florence, Begum Dilara, Wing Rod A., Glaszmann Jean-Christophe.
2002. Exploitation of sorghum molecular for map-based cloning of a major rust resistance gene in sugarcane.
In : Plant, Animal and Microbe Genomes 10th Conference. Abstracts
Résumé : A rust resistance gene is used as a target to experience map-based cloning in a typical interspecific, highly polyploid, aneuploid sugarcane cultivar (Saccharum spp., 2n= 100 to 130). Bulk segregant analysis with AFLP markers allowed surrounding the target resistance gene with 8 AFLP markers, the closest being located at 2 cM on each side of the gene. These two AFLP markers were cloned and mapped on sorghum, suggesting a local inversion between sugarcane and sorghum. Several RFLP loci located in the target region in sorghum maps were then mapped on sugarcane. They allowed surrounding the gene with markers located at 0.3 and 0.6 cM on each side. These markers were also used to screen a partially ordered sorghum BAC library, enabling us to build a sorghum BAC contig covering the homeologous target region in sorghum. If synteny is conserved at a fine level in this region, this contig should also cover the region that encompasses the rust resistance gene in sugarcane.
Mots-clés Agrovoc : Sorghum, résistance aux maladies, Pucciniales, maladie fongique, gène, Saccharum, carte génétique, marqueur génétique
Classification Agris : F30 - Génétique et amélioration des plantes
Auteurs et affiliations
- D'Hont Angélique, CIRAD-CA-Canne à sucre (FRA)
- Le Cunff Loïc
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Garsmeur Olivier, CIRAD-CA-Canne à sucre (FRA)
ORCID: 0000-0001-8869-3689
- Paulet Florence, CIRAD-CA-Canne à sucre (FRA)
- Begum Dilara, Clemson University (USA)
- Wing Rod A., Clemson University (USA)
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Glaszmann Jean-Christophe, CIRAD-AMIS-BIOTROP (FRA) ORCID: 0000-0001-9918-875X
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/487404/)
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