Grivet Laurent, Glaszmann Jean-Christophe, Arruda Paulo.
2002. Detection of SNP from EST data in sugarcane.
In : Plant, Animal and Microbe Genomes 10th Conference. Abstracts
Résumé : Discovering Single Nucleotide Polymorphism (SNP) thanks to Expressed Sequence Tags (ESTs) has proven to be valuable for the human genes, however this approach is not yet documented in plants. A large collection of more than 250 000 ESTs has been constituted for sugarcane by a network of Brazilian laboratories (SUCEST project; http://sucest.lad.ic.unicamp.br/en/). As sugarcane is highly polyploid and heterozygous, several alleles are expected to be present among the redundant sequence tags of each given gene, making this EST database a potential source for SNP discovery. A detailed study of sequence polymorphism is presented here for several genes in order to test the relevance of this approach. Perspectives for more automated large-scale detection methods, as well as perspective for using this information are discussed in the particular context of polyploid sugarcane.
Mots-clés Agrovoc : Saccharum, polymorphisme génétique, gène
Classification Agris : F30 - Génétique et amélioration des plantes
Auteurs et affiliations
- Grivet Laurent, CIRAD-CA-Canne à sucre (BRA)
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Glaszmann Jean-Christophe, CIRAD-AMIS-BIOTROP (FRA)
ORCID: 0000-0001-9918-875X
- Arruda Paulo, CBMEG (BRA)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/487406/)
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