Breitler Jean-Christophe, Royer Monique, Vassal Jean-Michel, Messeguer Joaquima, Marfa Victoria, Del Mar Catala Maria, San Segundo Blanca, Martinez Izquierdo Jose-Antonio, Meynard Donaldo, Guiderdoni Emmanuel.
2002. Transgenic Bt rice expressing a synthetic cry 1B gene : expression strategies and field protection against the striped stem borer : expression strategies and field protection against the striped stem borer.
In : International Colloquium on Invertebrate Pathology and Microbial Control, Foz do Iguassu, Brazil, 18-23 August 2002 : Proceedings. SIP, EMBRAPA-Soja, EMBRAPA-Recursos Genéticos e Biotecnologia, UEL, SEB
Résumé : We investigated whether the conditional, wound-inducible production of a Bacillus thuringiensis (Bt) Cry endotoxin may provide a hopeful and efficient alternative of protection from stem borer damage to the high dosage, constitutive or tissue-specific toxin delivery approach generally used in transgenic Bt crops. A synthetic cry1B gene controlled by the promoter region of either the constitutive ubiquitin (ubi) gene or of a wound-inducible proteinase inhibitor (mpi) gene from maize was introduced in rice by microprojectile bombardment. Accumulation of Cry1B from undetectable level to 0.2% of total soluble protein, 8-12 hours after mechanical wounding, was demonstrated in leaf and pith tissues of lines harbouring the pmpi-cry1B construct. In these plants, Cry1B was not detected in pollen and seed endosperm and embryo, unlike in pUbi-cry1B plants. Two out of 43 pmpi-cry1B plants were found to exhibit full protection against SSB damage. Field evaluation with artificial and natural infestations of the most promising ubi-cry1B and mpi-cry1B lines in Delta Del Ebre, Spain during the summer crop season 2001 confirmed the level of protection observed in greenhouse assays.
Mots-clés Agrovoc : Chilo suppressalis, lutte antiravageur, Oryza, plante transgénique, gène, Bacillus thuringiensis, expérimentation au champ
Classification Agris : H10 - Ravageurs des plantes
Auteurs et affiliations
- Breitler Jean-Christophe, CIRAD-AMIS-BIOTROP (FRA)
- Royer Monique, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
- Vassal Jean-Michel, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
- Messeguer Joaquima, IRTA (ESP)
- Marfa Victoria, IRTA (ESP)
- Del Mar Catala Maria, IRTA (ESP)
- San Segundo Blanca, IBMB-CSIC (ESP)
- Martinez Izquierdo Jose-Antonio, IBMB-CSIC (ESP)
- Meynard Donaldo, CIRAD-AMIS-BIOTROP (FRA)
- Guiderdoni Emmanuel, CIRAD-AMIS-BIOTROP (FRA)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/510411/)
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