Targeted mapping of a sugarcane rust resistance gene (Bru1) using bulked segregant analysis and AFLP markers

Asnaghi Carole, Roques Danièle, Ruffel Sandrine, Kaye Claudia, Hoarau Jean-Yves, Telismart Hugues, Girard Jean-Claude, Raboin Louis-Marie, Risterucci Ange-Marie, Grivet Laurent, D'Hont Angélique. 2004. Targeted mapping of a sugarcane rust resistance gene (Bru1) using bulked segregant analysis and AFLP markers. Theoretical and Applied Genetics, 108 (4) : 759-764.

https://doi.org/10.1007/s00122-003-1487-6

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Résumé : The presence of a major resistance gene ( Bru1) for brown rust in the sugarcane cultivar R570 (2n about 115) was confirmed by analyzing segregation of rust resistance in a large population of 658 individuals, derived from selfing of clone R570. A subset of this population was analyzed with AFLP and bulked segregant analysis (BSA) to develop a detailed genetic map around the resistance gene. Four hundred and forty three primer pairs were used resulting in the identification of eight AFLP markers surrounding the resistance gene in an interval of 10 cM, with the closest markers located at 1.9 and 2.2 cM on each side of the gene. Efficiency of the AFLP/BSA applied to the complex polyploid genome of sugarcane is discussed, as well as the potential of the newly identified AFLP markers for developing a map-based cloning approach exploiting, synteny conservation with sorghum.

Mots-clés Agrovoc : Saccharum, Puccinia melanocephala, maladie fongique, résistance aux maladies, expérimentation au champ, gène, marqueur génétique, ségrégation, traitement des données, méthode statistique

Mots-clés géographiques Agrovoc : La Réunion, France

Mots-clés complémentaires : AFLP

Classification Agris : F30 - Génétique et amélioration des plantes
H20 - Maladies des plantes

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