Screening and evaluation of SSR primers in gel systems for the detection of off-types in cocoa field genebanks

Motilal Lambert A., Boccara Michel. 2004. Screening and evaluation of SSR primers in gel systems for the detection of off-types in cocoa field genebanks. Ingenic newsletter (9) : 21-26.

Autre titre : Criblage et évaluation d'amorces SSR dans des systèmes gel pour la détection de variants dans des banques de gènes de cacaoyer au champ

Résumé : The set of SSRs recommended by J. Saunders, formerly of USDA, for verification purposes, i. e. unique identification of accessions in field genebanks, is discussed. We suggest that twelve primer pairs be a minimum for verification studies using gel systems as done at CRU. The selection of SSRs to be used is suggested as an issue requiring further discussion. Based on our studies, the mTcCIR primers 1, 6, 8, 11, 12, 15, 29, 42, 58, and 61 are proposed as ideal candidates for use in such verification studies.

Mots-clés Agrovoc : Theobroma cacao, microsatellite, collection de matériel génétique, identification, variant, méthode

Mots-clés géographiques Agrovoc : Trinité-et-Tobago

Mots-clés complémentaires : SSR

Classification Agris : F30 - Génétique et amélioration des plantes
U30 - Méthodes de recherche

Auteurs et affiliations

• Motilal Lambert A., UWI (TTO)
• Boccara Michel, CIRAD-CP-CACAO (TTO)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/522482/)

Métriques

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