Understanting the Theobroma cacao - Crinipellis perniciosa interaction using ESTs and proteomic analyses

Da Silva Gesteira Abelmon, Micheli Fabienne, Gramacho Karina Peres, Cerqueira Alvim Fatima, Priminho Pirovani Carlos, Leal Pires Acassia Benjamin, Mariano Andréa Cristina, Carels Nicolas, Pires José Luis, Vincentz Michel, Pereira G., Cascardo Julio Cezar M.. 2004. Understanting the Theobroma cacao - Crinipellis perniciosa interaction using ESTs and proteomic analyses. Ingenic newsletter (9) : 27-32.

Autre titre : Comprendre l'intéraction Theobroma cacao - Crinipellis perniciosa grâce aux EST et aux analyses protéomiques

Résumé : Using the tert-Butanol RNA extraction protocol, we succeeded, for the first time, in isolating RNA from cocoa organs infected by C. perniciosa. These results have led to new perspectives regarding the understanding, through genomic studies, of the cellular mechanisms of the infection of the cocoa tree by destructive pathogens such as Crinipellis or Phytophthora spp. The collection of ESTs we obtained will provide new data about the physiology of the interaction between Theobroma cacao and C. perniciosa. In the short term, we plan to do a functional analysis of some candidate genes, which can also be used for mapping, to facilitate introgression strategies and to create new varieties resistant to C. perniciosa. These data may also be related to research on gene expression of cocoa challenged with other pathogens, such as Phytophthora spp.

Mots-clés Agrovoc : Theobroma cacao, Crinipellis perniciosa, Champignon, relation hôte pathogène, technique analytique, arn, séquence nucléotidique, anticorps monoclonal, localisation de gène

Mots-clés géographiques Agrovoc : Brésil

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Da Silva Gesteira Abelmon, UESC (BRA)
• Micheli Fabienne, CIRAD-CP-CACAO (BRA)
• Gramacho Karina Peres, CEPLAC (BRA)
• Cerqueira Alvim Fatima, UESC (BRA)
• Priminho Pirovani Carlos, UESC (BRA)
• Leal Pires Acassia Benjamin, UESC (BRA)
• Mariano Andréa Cristina, UESC (BRA)
• Carels Nicolas, UESC (BRA)
• Pires José Luis, CEPLAC (BRA)
• Vincentz Michel, UNICAMP (BRA)
• Pereira G., UNICAMP (BRA)
• Cascardo Julio Cezar M., UESC (BRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/522484/)

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