Polymerase chain reaction-restriction fragment length polymorphism markers for the fungal banana pathogen Mycosphaerella fijiensis

Zapater Marie-Françoise, Rakotonantoandro Andry, Cohen S., Carlier Jean. 2004. Polymerase chain reaction-restriction fragment length polymorphism markers for the fungal banana pathogen Mycosphaerella fijiensis. Molecular Ecology Notes, 4 (1) : 80-82.

Résumé : Polymerase chain reactionrestriction fragment length polymorphism markers were developed to study populations of the fungal banana pathogen #Mycosphaerella fijiensis#. Twelve markers were defined, 11 in anonymous and single-copy nuclear DNA sequences and one in the internal transcribed spacer and 5.8S rDNA sequence. The polymerase chain reaction products obtained with locus-specific primer pairs were digested with restriction enzymes to reveal polymorphism. Between five and 12 markers were polymorph in #M. fijiensis# populations from different geographical origins (Papua New Guinea, the Philippines, Cameroon and Latin America). The mean of allele number and gene diversity (expected heterozygosity) per locus in the different geographical populations ranged between 1.4 and 2.7 and 0.17 and 0.45, respectively.

Mots-clés Agrovoc : Musa, banane, Mycosphaerella fijiensis, RFLP, maladie fongique, génétique des populations, PCR, marqueur génétique, polymorphisme génétique, ressource génétique

Mots-clés géographiques Agrovoc : Papouasie-Nouvelle-Guinée, Philippines, Cameroun, Amérique latine

Classification Agris : F30 - Génétique et amélioration des plantes
H20 - Maladies des plantes

Auteurs et affiliations

• Zapater Marie-Françoise, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
• Rakotonantoandro Andry, CIRAD-AMIS-UMR BGPI (FRA)
• Cohen S., CIRAD-AMIS-UMR BGPI (FRA)
• Carlier Jean, CIRAD-AMIS-PROTECTION DES CULTURES (FRA) ORCID: 0000-0002-6967-1852

Source : Cirad - Agritrop (https://agritrop.cirad.fr/522586/)

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