Medfly microsatellite markers for species diagnosis and population genetic analysis in three other fruit fly (Diptera : Tephritidae) species

Baliraine F.N., Bonizzoni Mariangela, Osir E.O., Lux Slawomir A., Mulaa F.J., Quilici Serge, Gomulski L.M., Gasperi Giuliano, Malacrida Anna R.. 2002. Medfly microsatellite markers for species diagnosis and population genetic analysis in three other fruit fly (Diptera : Tephritidae) species. In : Proceedings of the 6th International Symposium on Fruit Flies of Economic Importance, 6-10 May 2002, Stellenbosch, South Africa. Barnes Brian N. (ed.). ARC. Irene : Isteg Scientific Publications, 411-414. ISBN 1-86849-298-2 International Symposium on Fruit Flies of Economic Importance. 6, Stellenbosch, Afrique du Sud, 6 Mai 2002/10 Mai 2002.

Résumé : Tephritid fruit flies of the genus Ceratitis MacLeay (Dacinae: Ceratitidini) pose one of the greatest threats to Africa's horticultural industry. To curb their economic injury and prevent spread to new areas, it is vital that quarantine workers and developers of control tools and strategies can unambiguously identify the different species atany life age. However, the classification ofTephritidae to the species level based on morphology alone continues to be difficult. We have sought to utilize molecular markers, which are life-stage independent, stable and intrinsic to the organisms, for this purpose. Consequently, we screened 24 Medfly (Ceratitis capitata) microsatellite markers for cross-species amplification in C. rosa, C. fasciventris and C. cosyra. The presence or absence of each locus in each species was tested by PCR, and negative results confirmed by Southern analysis. Locus homology species was tested by PCR, and negative result confirmed by sequencing. Our findings indicate that is is possible to distinguish the different species examine using a combination of the medfly-bases primers. Of the 24 loci screene, 17 were common to all, five were absent in C. Cosyra, three were absent in C. Rosa, two were absent in C. Fasciventris, while on locus was present only in C. capitata. In addition, sequence results indicate that most of the medfly-based microsatellite markers could be useful for population genetic studies in the species tested, an aspect wich would facilitate the tracing of the geographical origin of colonist pest populations, assessment of their invasive potential and risk assessment.

Mots-clés Agrovoc : culture fruitière, Tephritidae, gène, marqueur génétique, diagnostic, PCR, Ceratitis

Mots-clés complémentaires : Ceratitis rosa, Mouche des fruits

Classification Agris : L10 - Génétique et amélioration des animaux
H10 - Ravageurs des plantes

Auteurs et affiliations

• Baliraine F.N., ICIPE (KEN)
• Bonizzoni Mariangela, Universita di Pavia (ITA)
• Osir E.O., ICIPE (KEN)
• Lux Slawomir A., ICIPE (KEN)
• Mulaa F.J., University of Nairobi (KEN)
• Quilici Serge, CIRAD-FLHOR-ARF (REU)
• Gomulski L.M., Universita di Pavia (ITA)
• Gasperi Giuliano, Universita di Pavia (ITA)
• Malacrida Anna R., Universita di Pavia (ITA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/524466/)

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