A hydroponic culture system for growing Arabidopsis thaliana plantlets under sterile conditions

Schlesier Bernhard, Breton Frédéric, Mock Hans-Peter. 2003. A hydroponic culture system for growing Arabidopsis thaliana plantlets under sterile conditions. Plant Molecular Biology Reporter, 21 (4) : 449-456.

Résumé : We developed a hydroponic cultivation system for growing Arabidopsis plantlets under sterile, controlled environmental conditions. The system consists of a piece of stainless-steel wire cloth (125 [mu]m mesh size) that is fixed between 2 flat rings and held in place by 3 legs, placed in a commercially-available glass jar, and covered by the original glass lid or a sheet of sterilized cellophane. Sterilized seeds were distributed evenly across the mesh piece, the size of which allowed root growth and kept the seeds in place. After 3 weeks of cultivation, shoot and root tissues were easily harvested without mechanical damage. Proteome and metabolite analyses were performed on root and shoot tissues and demonstrated excellent reproducibility, indicating that the system is advantageous when biological variation is minimized. Induction experiments can be performed by transferring the apparatus (with plants) to a new jar containing a different nutrient solution. The apparatus is reusable and can easily be sterilized by autoclaving or dry heat. The system can be adapted to other small-seed plants by varying the mesh size.

Mots-clés Agrovoc : Arabidopsis thaliana, substrat de culture, culture hydroponique, contrôle du milieu, analyse de tissus

Classification Agris : F60 - Physiologie et biochimie végétale

Auteurs et affiliations

• Schlesier Bernhard, Leibniz Institute of Plant Genetics and Crop Plant Research (DEU)
• Breton Frédéric, CIRAD-CP-PALMIER (IDN)
• Mock Hans-Peter, Leibniz Institute of Plant Genetics and Crop Plant Research (DEU)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/525182/)

Métriques

[ Page générée et mise en cache le 2024-04-26 ]