Molecular characterization of new members of the Hevea brasiliensis hevein multigene family and analysis of their promoter region in rice

Pujade-Renaud Valérie, Sanier Christine, Cambillau Laurence, Pappusamy Arokiaraj, Jones Heddwyn, Ruengsri Natsuang, Tharreau Didier, Chrestin Hervé, Montoro Pascal, Narangajavana Jarunya. 2005. Molecular characterization of new members of the Hevea brasiliensis hevein multigene family and analysis of their promoter region in rice. Biochimica and Biophysica Acta, N. Gene structure and Expression, 1727 (3) : 151-161.

https://doi.org/10.1016/j.bbaexp.2004.12.013

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Résumé : The cloning of hevein genes from Hevea brasiliensis was undertaken with the objective to isolate useful promoters to drive transgene expression in genetically engineered rubber tree. Four different full length genes were cloned by library screening and a fifth, a partial gene, by adaptor-anchored PCR. Sequence alignment revealed that hevein genes, although highly conserved in their transcribed region, diverged in two groups, with major differences in their promoter region, suggesting a more rapid evolution of the upstream regulatory functions of the genes than the downstream functions of their protein products. The promoter regions from two hevein genes representative of each group were isolated and analyzed in rice. Although both were functional, only the longest promoter sequence (PHev2.1) conferred a high level of expression to the transgene in various tissues of this heterologous host. It was in addition up-regulated by mechanical wounding and fungal infection in leaves. A number of potential cis-regulatory elements were identified in silico and are discussed in view of the expression profiles observed in rice.

Mots-clés Agrovoc : Hevea brasiliensis, Oryza sativa, stress, transfert de gène, expression des gènes, Clonage moléculaire, génie génétique

Mots-clés complémentaires : Promoteur, Héveine

Classification Agris : F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

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