New media for the semiselective isolation and enumeration of Xanthomonas campestris pv. mangiferaeindicae, the causal agent of mango bacterial black spot

Pruvost Olivier, Roumagnac Philippe, Gaube C., Chiroleu Frédéric, Gagnevin Lionel. 2005. New media for the semiselective isolation and enumeration of Xanthomonas campestris pv. mangiferaeindicae, the causal agent of mango bacterial black spot. Journal of Applied Microbiology, 99 (4) : 803-815.

https://doi.org/10.1111/j.1365-2672.2005.02681.x

Article de revue ; Article de revue à facteur d'impact

Version publiée - Anglais
Accès réservé aux personnels Cirad
Utilisation soumise à autorisation de l'auteur ou du Cirad.
document_528534.pdf
Télécharger (266kB)

Résumé : Aims: Mango bacterial black spot, caused by Xanthomonas campestris pv. mangiferaeindicae, is a potentially severe disease in several tropical and subtropical areas. Data describing the life cycle of the pathogen are needed for improving integrated pest management strategies. Because of the important bacterial microflora associated with mango leaves, isolation of the pathogen is often difficult using nonselective agar media. Methods and Results: A previously developed medium, BVGA, failed to inhibit several Gram-negative saprophytic bacteria, especially those belonging to Enterobacteriaceae. Two new semiselective media were developed. The selectivity of KC and NCTM3 media was achieved using cephalexin 40 mg 1-1, kasugamycin 20 mg 1-1 and neomycin 1 mg 1-1, cephalexin 100 mg 1-1, trimethoprime 5 mg 1-1, pivmecillinam 100 mg 1-1 respectively. Plating efficiencies ranged from 76 to 104% and from 78 to 132% for KC and NCTM3 respectively. Conclusions: The new media allowed the growth of X. campestris pv. mangiferaeindicae whatever its country of isolation. The pathogen was repeatedly isolated with these media from asymptomatic leaves sampled in growth chamber experiments. Significance and Impact of the Study: This work provides a description of new semiselective media, which should be valuable tools to study the ecology and epidemiology of X. campestris pv. mangiferaeindicae.

Mots-clés Agrovoc : mangue, fruit (botanique), maladie bactérienne, Xanthomonas, agent pathogène, identification, épidémiologie, lutte intégrée, zone tropicale, zone subtropicale

Mots-clés complémentaires : Xanthomonas campestris pv. mangiferaeindicae

Classification Agris : H10 - Ravageurs des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations