OryGenesDB: A database for rice reverse genetics

Droc Gaëtan, Ruiz M., Larmande Pierre, Pereira Andy, Piffanelli Pietro, Morel Jean-Benoit, Dievart Anne, Courtois Brigitte, Guiderdoni Emmanuel, Perin Christophe. 2006. OryGenesDB: A database for rice reverse genetics. Nucleic Acids Research, 34 : 736-740.

https://doi.org/10.1093/nar/gkj012

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Résumé : Insertional mutant databases containing Flanking Sequence Tags (FSTs) are becoming key resources for plant functional genomics. We have developed OryGenesDB (http://orygenesdb.cirad.fr/), a database dedicated to rice reverse genetics. Insertion mutants of rice genes are catalogued by Flanking Sequence Tag (FST) information that can be readily accessed by this database. Our database presently contains 44166 FSTs generated by most of the rice insertional mutagenesis projects. The OryGenesDB genome browser is based on the powerful Generic Genome Browser (GGB) developed in the framework of the Generic Model Organism Project (GMOD). The main interface of our web site displays search and analysis inter-faces to look for insertions in any candidate gene of interest. Several starting points can be used to exhaustively retrieve the insertions positions and associated genomic information using blast, keywords or gene name search. The toolbox integrated in our database also includes an 'anchoring' option that allows immediate mapping and visualization of up to 50 nucleic acid sequences in the rice Genome Browser of OryGenesDB. As a first step toward plant comparative genomics, we have linked the rice and Arabidopsis whole genome using all the predicted pairs of orthologs by best BLAST mutual hit (BBMH) connectors.

Mots-clés Agrovoc : Oryza sativa, riz, banque de gènes, mutant, génétique moléculaire, génome, pool de gènes

Mots-clés complémentaires : Réversion, Gène d'intérêt

Classification Agris : F30 - Génétique et amélioration des plantes
C30 - Documentation et information

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

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