Physical delimitation of the pepper Bs3 resistance gene specifying recognition of the AvrBs3 protein from Xanthomonas campestris pv. vesicatoria

Jordan Tina, Römer Patrick, Meyer Annett, Szczesny Robert, Pierre Michèle, Piffanelli Pietro, Bendahmane Abdel, Bonas Ulla, Lahaye Thomas. 2006. Physical delimitation of the pepper Bs3 resistance gene specifying recognition of the AvrBs3 protein from Xanthomonas campestris pv. vesicatoria. Theoretical and Applied Genetics, 113 (5) : 895-905.

Résumé : The pepper (Capsicum annuum) Bs3 gene confers resistance to avrBs3-expressing strains of the bacterial spot pathogen Xanthomonas campestris pv. vesicatoria. To physically delimit Bs3, a pepper YAC library was screened with two flanking DNA markers that are separated from Bs3 by 1.0 and 1.2 cM, respectively resulting in the identification of three YAC clones. Genetic mapping of the corresponding YACends revealed however, that these YACs do not cover Bs3 and subsequent screens with newly developed YACend markers failed to identify new YAC clones. Marker saturation at the Bs3 locus was carried out by amplified fragment length polymorphism (AFLP). The analysis of 1,024 primer combinations resulted in the identification of 47 new Bs3-linked AFLPs. High-resolution linkage mapping of Bs3 was accomplished by inspecting more than 4,000 F2 segregants resulting in a genetic resolution of 0.01 cM. Using tightly Bs3-linked YACend- and AFLP-derived markers we established a Bs3-spanning BAC contig and physically delimited the target gene within one BAC clone. The analysis of the Bs3-containing genomic region revealed substantial local variation in the correlation of genetic and physical distances.

Mots-clés Agrovoc : Capsicum annuum, localisation de gène, marqueur génétique, PCR, identification, séquence nucléotidique, testage

Mots-clés complémentaires : AFLP, Xanthomonas vesicatoria

Classification Agris : F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Jordan Tina, Martin Luther University (DEU)
• Römer Patrick, Martin Luther University (DEU)
• Meyer Annett, Martin Luther University (DEU)
• Szczesny Robert, Martin Luther University (DEU)
• Pierre Michèle, CNRS (FRA)
• Piffanelli Pietro, CIRAD-AMIS-UMR PIA (FRA)
• Bendahmane Abdel, INRA (FRA)
• Bonas Ulla, Martin Luther University (DEU)
• Lahaye Thomas, Martin Luther University (DEU)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/536023/)

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