Utilization of chromosome painting as a complementary Tool for introgression analysis and chromosome identification in coffee

Herrera Juan Carlos, D'Hont Angélique, Lashermes Philippe. 2007. Utilization of chromosome painting as a complementary Tool for introgression analysis and chromosome identification in coffee. In : 21st International Conference on Coffee Science, Montpellier (France), 11th - 15th September 2006 = 21ème Colloque scientifique international sur le café ; 21. Internationales Wissenshaftliches Kolloquium über Kaffee ; 21e Coloquio Cientifico Internacional sobre el Café. ASIC. Montpellier : ASIC, 660-664. ISBN 2-900212-20-0 Colloque Scientifique International sur le Café. 21, Montpellier, France, 11 Septembre 2006/15 Septembre 2006.

Résumé : The term "chromosome painting" widely implies painting of differential chromosome segments with sequence specific probes based on the technique of in situ molecular hybridization. Development of fluorescence in situ hybridization (FISH), further enhanced sensitivity and versatility of in situ hybridization procedures. Despite recent development and application of FISH in plant genome analysis, this technology remains unfamiliar to most coffee scientists. Here we report through of different examples, the potential of FISH technique as a tool for genome analysis in coffee. We investigated the presence of alien chromatin in interspecific hybrids between C. arabica x C. canephora as well as in one C. arabica line introgressed from C. liberica. Further, we demonstrated that it is possible to identify one specific chromosome on the whole genome, despite the morphological similarity between coffee chromosomes. Overall, our results illustrate how molecular cytogenetics approach would provide complementary information for genetic mapping studies based on molecular markers.

Mots-clés Agrovoc : Coffea arabica, Coffea canephora, Coffea liberica, hybridation interspécifique, hybridation moléculaire, carte génétique, fluorescence, marqueur génétique, génome, technique analytique, cytogénétique, biologie moléculaire

Classification Agris : F30 - Génétique et amélioration des plantes
F70 - Taxonomie végétale et phytogéographie

Auteurs et affiliations

• Herrera Juan Carlos, CENICAFE (COL)
• D'Hont Angélique, CIRAD-AMIS-UMR PIA (FRA)
• Lashermes Philippe, IRD (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/540055/)

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