Variability in the insecticidal protein concentration within transformed Coffea canephora observed in a field experiment

Perthuis Bernard, Leroy Thierry, Dufour Magali, Vassal Jean-Michel, Pradon Jean-Louis, Fenouillet Catherine, Condina Virgile, Laurent Sylvain. 2007. Variability in the insecticidal protein concentration within transformed Coffea canephora observed in a field experiment. In : 21st International Conference on Coffee Science, Montpellier (France), 11th - 15th September 2006 = 21ème Colloque scientifique international sur le café ; 21. Internationales Wissenshaftliches Kolloquium über Kaffee ; 21e Coloquio Cientifico Internacional sobre el Café. ASIC. Montpellier : ASIC, 1390-1393. ISBN 2-900212-20-0 Colloque Scientifique International sur le Café. 21, Montpellier, France, 11 Septembre 2006/15 Septembre 2006.

Résumé : A field trial of transformed Coffea canephora for the synthesis of the Bt protein cry1Ac against coffee South-American leaf miner was performed in French Guiana. Almost all the transformed clones were highly resistant against Leucoptera coffeella. The strategy of plant selection requires the creation of lines synthesizing large amounts of insecticidal protein. The transgenic protein in the leaves of 15 independent transformed clones was quantified once. A significant variability in the transgenic protein concentration was seen among the clones. The potential of the used genetic construct seems interesting.

Mots-clés Agrovoc : Coffea canephora, expérimentation au champ, plante transgénique, Bacillus thuringiensis, insecticide, Leucoptera, résistance aux organismes nuisibles, protéine, concentration

Mots-clés complémentaires : Leucoptera coffeella

Classification Agris : F30 - Génétique et amélioration des plantes
H10 - Ravageurs des plantes

Auteurs et affiliations

• Perthuis Bernard, CIRAD-AMIS-UMR PIA (GUF)
• Leroy Thierry, CIRAD-AMIS-UMR PIA (FRA)
• Dufour Magali, CIRAD-AMIS-UMR PIA (FRA)
• Vassal Jean-Michel, CIRAD-AMIS-UPR Acridologie (FRA)
• Pradon Jean-Louis, CIRAD-CP-US Services en Guyane (GUF)
• Fenouillet Catherine, CIRAD-CP-UPR Bioagresseurs de pérennes (FRA)
• Condina Virgile, CIRAD-CP-UPR Bioagresseurs de pérennes (GUF)
• Laurent Sylvain, CIRAD-CP-US Services en Guyane (GUF)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/540134/)

Métriques

[ Page générée et mise en cache le 2024-03-29 ]