Effect of exogenous calcium on post-thaw growth recovery and subsequent plant regeneration of cryopreserved embryogenic calli of Hevea brasiliensis (Müll.Arg.)

Lardet Ludovic, Martin Florence, Dessailly Florence, Carron Marc-Philippe, Montoro Pascal. 2007. Effect of exogenous calcium on post-thaw growth recovery and subsequent plant regeneration of cryopreserved embryogenic calli of Hevea brasiliensis (Müll.Arg.). Plant Cell Reports, 26 (5) : 559-569.

Résumé : A reliable cryopreservation technique was developed for friable embryogenic callus lines of Hevea brasiliensis. The study showed that reducing the CaCl2 concentration of the pre-culture medium from 9 mM to 1 or 0 mM CaCl2 before cryopreservation promoted post-thaw callus growth, 1 mM being the optimum CaCl2 concentration for embryo regeneration. Post-thaw callus proliferation decreased in line with the increase of plated callus weight. The effect of cryopreservation was assessed on 39 independent lines showing that cryopreservation did not affect embryogenic and plant regeneration for a majority of lines. The decrease in CaCl2 concentration of the pre-culture medium led to a drop in callus calcium content indicating a direct link between the CaCl2 concentration of the pre-culture medium and the endogenous calcium content of the calli. It also highlighted the implication of tissue calcium content in cryotolerance. Callus water status and the different ways by which calcium could prevent cryoinjury is also discussed.

Mots-clés Agrovoc : Hevea brasiliensis, régénération in vitro, cal, croissance, multiplication végétative, embryon somatique, culture d'embryon, congélation, calcium

Classification Agris : F02 - Multiplication végétative des plantes
F62 - Physiologie végétale - Croissance et développement

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Lardet Ludovic, CIRAD-CP-UMR BEPC (FRA)
• Martin Florence, CIRAD-CP-UMR BEPC (FRA)
• Dessailly Florence, CIRAD-CP-UMR BEPC (FRA) ORCID: 0000-0001-7873-3974
• Carron Marc-Philippe, CIRAD-CP-UMR BEPC (FRA)
• Montoro Pascal, CIRAD-CP-UMR BEPC (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/540457/)

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