Microsatellite markers for population studies of the rice blast fungus, Magnaporthe grisea

Adreit Henri, Santoso, Andriantsimialona Dodelys, Utami Dwinita, Nottéghem Jean-Loup, Lebrun Marc-Henri, Tharreau Didier. 2007. Microsatellite markers for population studies of the rice blast fungus, Magnaporthe grisea. Molecular Ecology Notes, 7 (4) : 667-670.

Résumé : We developed nine new microsatellite markers for rice blast (Magnaporthe grisea) population studies. These markers were used in addition to nine microsatellite markers previously developed by our group for mapping purpose. Altogether, the 18 markers were used in multiplex PCR (polymerase chain reaction) to characterize six populations from different geographical origins. The average number of alleles per locus across populations ranged from 1.2 to 7 and the total number of alleles detected from 2 to 19. Based on this large range of polymorphism, this set of markers is expected to be useful for different kind of population studies at different geographical scales.

Mots-clés Agrovoc : Magnaporthe grisea, Oryza sativa, marqueur génétique, microsatellite, PCR, provenance, génétique des populations

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Adreit Henri, CIRAD-AMIS-UMR BGPI (FRA)
• Santoso, Research Institute for Rice (IDN)
• Andriantsimialona Dodelys, CENDRADERU (MDG)
• Utami Dwinita, RIFCB (IDN)
• Nottéghem Jean-Loup, ENSAM [Ecole nationale supérieure agronomique de Montpellier] (FRA)
• Lebrun Marc-Henri, CNRS (FRA)
• Tharreau Didier, CIRAD-AMIS-UMR BGPI (FRA) ORCID: 0000-0003-3961-6120

Source : Cirad - Agritrop (https://agritrop.cirad.fr/540786/)

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