Long-term storage at tropical temperature of dried-blood filter paper for detection and genotyping of RNA and DNA viruses by direct PCR

Michaud Vincent, Gil Patricia, Kwiatek Olivier, Prome Sylvie, Dixon Linda K., Romero Luis, Le Potier Marie-Frédérique, Arias M., Couacy-Hymann Emmanuel, Roger François, Libeau Geneviève, Albina Emmanuel. 2007. Long-term storage at tropical temperature of dried-blood filter paper for detection and genotyping of RNA and DNA viruses by direct PCR. Journal of Virological Methods, 146 : 257-265.

Résumé : In tropical countries the diagnosis of viral infections of humans or animals is often hampered by the lack of suitable clinical material and the necessity to maintain a cold chain for sample preservation up to the laboratory. This study describes the use of filter papers for rapid sample collection, and the molecular detection and genotyping of viruses when stored over long periods at elevated temperatures. Infected blood was collected on filter papers, dried and stored at different temperatures (22, 32 and 37°C) for various periods (up to 9 months). Two animal viruses, African swine fever, a large double-stranded DNA virus and Peste des Petits Ruminants, a negative single-stranded RNA virus, were used to validate the method. Filter papers with dried blood containing virus or control plasmid DNA were cut in small 5 mm2 pieces and added directly to the PCR tube for conventional PCR. Nucleic acid from both viruses could still be detected after 3 months at 32°C. Moreover, the DNA virus could be detected at least 9 months after conservation at 37°C. PCR products obtained from the filter papers were sequenced and phylogenetic analysis carried out. The results were consistent with published sequences, demonstrating that this method can be used for virus genotyping. © 2007 Elsevier B.V. All rights reserved.

Mots-clés Agrovoc : PCR, génétique moléculaire, phylogénie, peste porcine africaine, peste des petits ruminants

Classification Agris : L50 - Physiologie et biochimie animales
L73 - Maladies des animaux

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

• Michaud Vincent, CIRAD-EMVT-UPR Contrôle des maladies (FRA)
• Gil Patricia, CIRAD-EMVT-UPR Contrôle des maladies (FRA) ORCID: 0000-0001-7984-7340
• Kwiatek Olivier, CIRAD-EMVT-UPR Contrôle des maladies (FRA)
• Prome Sylvie, CIRAD-EMVT-UPR Contrôle des maladies (FRA)
• Dixon Linda K., Institute for Animal Health (GBR)
• Romero Luis, CISA (ESP)
• Le Potier Marie-Frédérique, AFSSA (FRA)
• Arias M., LANADA (CIV)
• Couacy-Hymann Emmanuel, LANADA (CIV)
• Roger François, CIRAD-EMVT-UPR Epidémiologie (FRA) ORCID: 0000-0002-1573-6833
• Libeau Geneviève, CIRAD-EMVT-UPR Contrôle des maladies (FRA)
• Albina Emmanuel, CIRAD-EMVT-UPR Contrôle des maladies (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/541964/)

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