Molecular analysis of a full-length sequence of a new yam badnavirus from Dioscorea sansibarensis

Seal Sue E., Muller Emmanuelle. 2007. Molecular analysis of a full-length sequence of a new yam badnavirus from Dioscorea sansibarensis. Archives of Virology, 152 (4) : 819-825.

Résumé : Badnavirus-like particles were observed by ISEM in viral preparations from yam (Dioscorea sansibarensis) leaves from Benin. Use of the viral preparation as template for PCR amplification with badnavirus-specific primers gave rise to a 579-bp product with most nucleotide identity (70.8%) to Dioscorea alata bacilliform virus (DaBV, Accession numbers X94575-X94582), the only other yam badnavirus sequenced to date. A full-length badnavirus sequence was generated, which consisted of 7261 nucleotides with a typical Badnavirus genome organisation. The full-length sequence shared most identity (61.9%) to DaBV (Accession numbers X94575-X94582) and hence represents a member of a new badnavirus species termed Dioscorea sansibarensis bacilliform virus (DsBV).

Mots-clés Agrovoc : Dioscorea, virus des végétaux, identification, PCR, séquence nucléotidique

Mots-clés complémentaires : Badnavirus

Classification Agris : H20 - Maladies des plantes
U30 - Méthodes de recherche

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Seal Sue E., NRI (GBR)
• Muller Emmanuelle, CIRAD-AMIS-UMR BGPI (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/542534/)

Métriques

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