Heterozygosity in diploid Musa lines: Implications for marker development, genomics, sequencing and breeding vegetatively propagated crops

Heslop Harrison Pat, Cheung Foo, Moskal William A., Hippolyte Isabelle, Baurens Franc-Christophe, Bakry Frédéric, Sasaki Takuji, Matsumoto Takashi, Rouard Mathieu, Schwarzacher Trude, Roux Nicolas, Town Christopher. 2008. Heterozygosity in diploid Musa lines: Implications for marker development, genomics, sequencing and breeding vegetatively propagated crops. In : Abstracts of Plant and Animal Genomes XVIth Conference, San Diego, CA (USA), January 12-16, 2008. s.l. : s.n., Résumé Plant and Animal Genomes Conference. 16, San Diego, États-Unis, 12 Janvier 2008/16 Janvier 2008.

Résumé : Musa species (bananas and plantains) are usually vegetatively propagated, and we have measured the levels and nature of heterozygosity within various accessions to examine genetic and genomic diversity, to compare heterozygosity with other species which are in-breeding, to investigate the feasibility of shot-gun sequencing approaches in potentially heterozygous plants, to optimise marker development, and to better characterize a single doubled-haploid Musa accession. We studied genic regions, areas flanking SSRs, and non-gene, non-SSR fragments of the genome. We analysed heterozygosity in single plants of various accessions of diploid banana (Musa) accessions by two approaches: PCR amplification of fragments of genomic DNA and sequencing, and by comparison of sequenced BACs from homologous chromosome regions from single plants. The study was carried out with a focus on Musa acuminata 'Calcutta 4', 'Pahang' and another a Musa malaccensis accession, and a doubled haploid line derived from 'Pahang', along with some sequences from M. schizocarpa and M. balbisiana. (Texte intégral)

Mots-clés Agrovoc : Musa

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Heslop Harrison Pat, University of Leicester (GBR)
• Cheung Foo, The J. Craig Venter Institute (USA)
• Moskal William A., The J. Craig Venter Institute (USA)
• Hippolyte Isabelle, CIRAD-BIOS-UPR Multiplication végétative (FRA)
• Baurens Franc-Christophe, CIRAD-BIOS-UMR DAP (FRA) ORCID: 0000-0002-5219-8771
• Bakry Frédéric, CIRAD-BIOS-UPR Multiplication végétative (FRA)
• Sasaki Takuji, NIAS (JPN)
• Matsumoto Takashi, NIAS (JPN)
• Rouard Mathieu, Bioversity International (ITA)
• Schwarzacher Trude, University of Leicester (GBR)
• Roux Nicolas
• Town Christopher, The J. Craig Venter Institute (USA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/545524/)

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