In vitro comparisons between Carica papaya and pancreatic lipases during test meal lipolysis : potential use of CPL in enzyme replacement therapy

Abdelkafi Slim, Fouquet Benjamin, Barouh Nathalie, Durner Sophie, Pina Michel, Scheirlinckx Frantz, Villeneuve Pierre, Carrière Frédéric. 2009. In vitro comparisons between Carica papaya and pancreatic lipases during test meal lipolysis : potential use of CPL in enzyme replacement therapy. Food Chemistry, 115 : 488-494.

https://doi.org/10.1016/j.foodchem.2008.12.043

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Quartile : Q1, Sujet : FOOD SCIENCE & TECHNOLOGY / Quartile : Q1, Sujet : CHEMISTRY, APPLIED / Quartile : Q1, Sujet : NUTRITION & DIETETICS

Résumé : High levels of lipase activity are known to occur in Carica papaya latex, and this activity is being used in some biotechnological applications. The lipolytic activity of C. papaya lipase (CPL) on dietary triacylglycerols (TAG) has not yet been studied. Hence, the aim of this study was to characterise the specific activity of CPL on dietary TAG present in a crude preparation. Also, we have determined its stability during the lipolysis of a test meal at various pH values mimicking those occurring in the gastro-intestinal tract, with or without bile, and have compared these properties with those of porcine pancreatic extract (PPE) and human pancreatic lipase (HPL). CPL showed maximum stability at pH 6.0, both with and without bile. Some residual activity was still observed at pH 2 (20%), whereas the pancreatic lipases tested were immediately completely inactivated at this pH. In the absence of bile, the highest specific activities were measured at pH 6 in the case of CPL, PPE and HPL. Adding bile slightly decreased the CPL activity in the 4-6 pH range, thus shifting the optimum CPL activity to pH 7, where the presence of bile had no effect. Lipolysis levels decreased with the pH, but CPL was still more active than PPE at pH 5 on a relative basis. These results suggest that CPL might be a promising candidate for use as a therapeutic tool on patients with pancreatic exocrine insufficiency.

Mots-clés Agrovoc : Carica papaya, latex, triacylglycérol lipase, lipase

Classification Agris : S20 - Physiologie de la nutrition humaine

Champ stratégique Cirad : Axe 3 (2005-2013) - Alimentation accessible et de qualité

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