Single-chain variable fragments antibody specific to Corynespora cassiicola toxin, cassiicolin, reduces necrotic lesion formation in Hevea brasiliensis

Sunderasan E., Kadir Rusni A., Pujade-Renaud Valérie, De Lamotte Frédéric, Yeang Hoong Yeet, Nathan Sheila. 2009. Single-chain variable fragments antibody specific to Corynespora cassiicola toxin, cassiicolin, reduces necrotic lesion formation in Hevea brasiliensis. Journal of General Plant Pathology, 75 : 19-26.

Résumé : Corynespora leaf disease poses a serious threat to rubber cultivation because infected leaves develop necrotic lesions and abscise, leaving the tree unproductive. The destructiveness of Corynespora cassiicola has been largely attributed to cassiicolin, a protein toxin secreted by the fungus. Recombinant antibody technology offers hope to curtail the disease whereby single-chain variable fragments (scFv) specific to cassiicolin could bind and deactivate the toxin in genetically modified rubber trees that harbour the antibody gene. A scFv phage library was constructed from heavy and light variable chains of IgG from cassiicolin immunized Balb/C mice spleen. Biopanning of the phage library yielded a scFv clone with high specificity to cassiicolin. The nucleotide sequence and deduced amino acid sequence information of the scFv were obtained. Hemagglutinin (HA)-tagged scFv expressed in Escherichia coli is discerned as a band at ca. 30 kDa on SDS-PAGE, and the corresponding band was detected by anti-HA IgG on a Western immunoblot. Deactivation of cassiicolin by the affinity-purified scFv was demonstrated in a detached-leaf bio-assay on selected susceptible Hevea clones (PB 260, RRIM 2020, RRIM 901 and RRIM 929). The assay was also performed on clones that are relatively more resistant to the fungus (RRIM 600 and GT-1), and the results were as expected. Thus, we have successfully demonstrated that the cassicolin-specific scFv can effectively reduce cassicolin toxicity.

Mots-clés Agrovoc : Hevea brasiliensis, Corynespora cassiicola, génie génétique, toxine, protéine microbiologique, glycoprotéine, réaction antigène anticorps, mécanisme de défense, anticorps, protéine recombinante

Mots-clés complémentaires : Cassiicoline

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Sunderasan E., RRIM (MYS)
• Kadir Rusni A., Universiti Kebangsaan Malaysia (MYS)
• Pujade-Renaud Valérie, CIRAD-BIOS-UMR DAP (FRA)
• De Lamotte Frédéric, INRA (FRA)
• Yeang Hoong Yeet, RRIM (MYS)
• Nathan Sheila, Universiti Kebangsaan Malaysia (MYS)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/548403/)

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