Genetic mapping of Magnaporthe grisea avirulence gene corresponding to leaf and panicle blast resistant QTLs in Jao Hom Nin Rice cultivar

Sreewongchai Tanee, Sriprakhon Siangchai, Vanavichit Apichart, Toojinda Theerayut, Tharreau Didier, Sirithunya Pattama. 2009. Genetic mapping of Magnaporthe grisea avirulence gene corresponding to leaf and panicle blast resistant QTLs in Jao Hom Nin Rice cultivar. Journal of Phytopathology, 157 (6) : 338-343.

Quartile : Q3, Sujet : PLANT SCIENCES

Résumé : The avirulence characteristic of Magnaporthe grisea isolate TH16 corresponding to Jao Hom Nin (JHN) rice cultivar was studied by mapping population of 140 random ascospore progenies derived from the cross between B1-2 and TH16 isolates. Segregation analyses of the avirulence characteristic performing on JHN rice at the seedling and flowering stages were performed in this mapping population. We used the reference map of Guy11/2539 to choose microsatellite DNA markers for mapping the avirulence gene. The genetic map of this population was constructed from 39-microsatellite markers. The genetic map was spanned by covering seven chromosomes with an average distance of 11.9 cM per marker. In mapping population the distribution of pathogenic and non-pathogenic progenies on JHN rice were found to be fitted to 1 : 1 ratio for two of the rice stages, seedling and flowering stages. The Quantitative Trait Loci (QTL) analysis for avirulence genes corresponding to two rice stages were located at the same region on chromosome 2 between markers Pyms305 and Pyms435. The LOD score and percentage of phenotypic variance explained (PVE) on two rice stages were 5.01/16.69 and 6.73/20.26, respectively. These loci were designated as Avr-JHN(lb) and Avr-JHN(pb) corresponding to leaf and panicle blast characteristics. The findings of this study can be the initial step for positional cloning and identifying any function of avirulence genes corresponding to leaf and panicle blast characteristics.

Mots-clés Agrovoc : Magnaporthe grisea, Oryza sativa, pouvoir pathogène, carte génétique, génétique des populations, marqueur génétique, locus des caractères quantitatifs, microsatellite, inflorescence, feuille, dépérissement

Mots-clés géographiques Agrovoc : Thaïlande

Mots-clés complémentaires : QTL

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Sreewongchai Tanee, BIOTEC [National Center for Genetic Engineering and Biotechnology] (THA)
• Sriprakhon Siangchai, BIOTEC [National Center for Genetic Engineering and Biotechnology] (THA)
• Vanavichit Apichart, BIOTEC [National Center for Genetic Engineering and Biotechnology] (THA)
• Toojinda Theerayut, BIOTEC [National Center for Genetic Engineering and Biotechnology] (THA)
• Tharreau Didier, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0003-3961-6120
• Sirithunya Pattama, Rajamangala University of Technology (THA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/549695/)

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