Sequence analysis of the large (L) polymerase gene and trailer of the peste des petits ruminants virus vaccine strain Nigeria 75/1: Expression and use of the L protein in reverse genetics

Minet Cécile, Yami Martha, Egziabher Berhe G., Gil Patricia, Tangy F., Bremont M., Libeau Geneviève, Diallo Adama, Albina Emmanuel. 2009. Sequence analysis of the large (L) polymerase gene and trailer of the peste des petits ruminants virus vaccine strain Nigeria 75/1: Expression and use of the L protein in reverse genetics. Virus Research, 145 (1) : 9-17.

https://doi.org/10.1016/j.virusres.2009.06.002

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Quartile : Q3, Sujet : VIROLOGY

Résumé : The large (L) polymerase gene and the 5_-terminal UTR of the genome of peste des petits ruminants virus (PPRV), vaccine strain Nigeria 75/1, were cloned and sequenced. The L protein was also expressed in eukaryotic cells and its polymerase activity was quantitatively measured in a PPR reverse genetics assay using a reporter minigenome.Comparative sequence analysis of this functional L gene with corresponding genes of other morbilliviruses showed a degree of conservation exceeding 70%. The multiple sequence alignment and the phylogenetic study of L gene discriminated the morbilliviruses in 6 clusters, which are more closely related to Tupaia and Henipaviruses than to other paramyxoviruses. Important protein domains and functional motifs of the L polymerase of the PPRV Nigeria 75/1 vaccine were also identified by using different bioinformatics tools.

Mots-clés Agrovoc : ruminant, morbillivirus, Paramyxoviridae, virus peste petits ruminants, vaccin, peste des petits ruminants, enzyme, petits ruminants

Mots-clés géographiques Agrovoc : Nigéria

Classification Agris : L73 - Maladies des animaux

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

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