Comparison of bacterial communities of tilapia fish from Cameroon and Vietnam using PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis)

Maïworé Justine, Tatsadjieu Ngoune Léopold, Montet Didier, Loiseau Gérard, Mbofung C.M.F.. 2009. Comparison of bacterial communities of tilapia fish from Cameroon and Vietnam using PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis). African Journal of Biotechnology, 8 (24) : 7156-7163.

Quartile : Q4, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Résumé : Fishes in general and tilapia in particular are traded all over the world. However, it is difficult to find out their exact geographical location. One of the techniques used in the traceability of fish and its byproducts consist in analysing in a global way the whole viable and non viable bacterial communities. For this purpose, the molecular technique employing the bacterial 16S DNA banding profiles generated by PCR-DGGE (polymerase chain reaction-Denaturing gradient gel electrophoresis) was used to evaluate the differences between the bacterial profiles of fishes from Vietnam (An Giang, south province) and those of Cameroon (Yagoua, Maga, Lagdo). The different PCR-DGGE 16S rDNA banding profiles obtained were analysed and results showed that there were specific bands for each geographical location though some bands common to Cameroon and Vietnam were observed. This method could be used as a rapid analytical traceability tool for fish products and could be considered as a provider of a unique biological bar code.

Mots-clés Agrovoc : Tilapia, flore microbienne, provenance, PCR, électrophorèse

Mots-clés géographiques Agrovoc : Cameroun, Viet Nam

Classification Agris : Q03 - Contamination et toxicologie alimentaires
M01 - Pêche et aquaculture - Considérations générales

Champ stratégique Cirad : Axe 3 (2005-2013) - Alimentation accessible et de qualité

Auteurs et affiliations

• Maïworé Justine, Université de Ngaoundéré (CMR)
• Tatsadjieu Ngoune Léopold, Université de Ngaoundéré (CMR)
• Montet Didier, CIRAD-PERSYST-UMR Qualisud (FRA)
• Loiseau Gérard, CIRAD-PERSYST-UMR Qualisud (FRA)
• Mbofung C.M.F., Université de Ngaoundéré (CMR)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/552840/)

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