Insulin-like growth factor-3 (IGF-3) in male and female gonads of the tilapia: Development and regulation of gene expression by growth hormone (GH) and 17a-ethinylestradiol (EE2)

Berishvili Giorgi, Baroiller Jean-François, Eppler Elisabeth, Reinecke Manfred. 2010. Insulin-like growth factor-3 (IGF-3) in male and female gonads of the tilapia: Development and regulation of gene expression by growth hormone (GH) and 17a-ethinylestradiol (EE2). General and Comparative Endocrinology, 167 (1) : 128-134.

Quartile : Q2, Sujet : ENDOCRINOLOGY & METABOLISM

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Résumé : Recently, in addition to IGF-1 and IGF-2 the existence of a third form of IGF, termed IGF-3, limited to fishes, to be present only in the gonads and encoded by a separate gene has been reported. However, no further data have been presented on IGF-3. The present study on tilapia (Oreochromis niloticus) uses quantitative real-time PCR specific for tilapia IGF-1 and IGF-3. The organ distribution of IGF-3 mRNA in adult fish and the early ontogeny of IGF-3 in male and female gonads were studied. The potential sensitivity of IGF-3 to GH was revealed by intraperitoneal injections of bream GH using IGF-1 as control gene. The effects of 17a-ethinylestradiol (EE2) exerted after feeding of high EE2 doses and exposure to low environmentally relevant EE2 doses on IGF-3 expression in testis and ovary during early development were determined. Low IGF-3 mRNA expression levels were detected in most organs studied, with the highest extra-gonadal amount in the pituitary. During development, the IGF-3 gene was significantly upregulated in male but downregulated in female gonad. Injections of GH elevated IGF-1 mRNA in male and female liver and ovary. IGF-3 did not respond to GH treatment neither in ovary nor in testis. Both EE2 treatments resulted in significant downregulations of IGF-3 mRNA in testis while ovarian IGF-3 mRNA did not respond. Thus, IGF-3 may be involved in reproduction of fishes most likely in the male gonad only. Whether IGF-3 also has some physiological significance in ovary or other organs should be the topic of further studies.

Mots-clés Agrovoc : Oreochromis niloticus, insuline, facteur de croissance, oestrogène, phylogénie, poisson osseux, ovaire, expression des gènes, somatotropine, régulation hormonale, développement biologique, physiologie de la reproduction

Classification Agris : L53 - Physiologie animale - Reproduction
M12 - Production de l'aquaculture

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Berishvili Giorgi, Institute of Anatomy (CHE)
• Baroiller Jean-François, CIRAD-PERSYST-UPR Aquaculture (FRA) ORCID: 0000-0002-0992-1725
• Eppler Elisabeth, Institute of Anatomy (CHE)
• Reinecke Manfred, Institute of Anatomy (CHE)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/554502/)

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