Expression and immunogenicity of the mycobacterial Ag85B/ESAT-6 antigens produced in transgenic plants by elastin-like peptide fusion strategy

Floss Doreen Manuela, Mockey Michael, Zanello Galliano, Brosson Damien, Diogon Marie, Frutos Roger, Bruel Timothée, Rodrigues Valérie, Garzon Edwin, Chevaleyre Claire, Berri Mustapha, Salmon Henri, Conrad Udo, Dedieu Laurence. 2010. Expression and immunogenicity of the mycobacterial Ag85B/ESAT-6 antigens produced in transgenic plants by elastin-like peptide fusion strategy. Journal of Biomedicine and Biotechnology (ID 274346), 14 p.

Quartile : Q3, Sujet : MEDICINE, RESEARCH & EXPERIMENTAL / Quartile : Q3, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Résumé : This study explored a novel system combining plant-based production and the elastin-like peptide (ELP) fusion strategy to produce vaccinal antigens against tuberculosis. Transgenic tobacco plants expressing the mycobacterial antigens Ag85B and ESAT-6 fused to ELP (TBAg-ELP) were generated. Purified TBAg-ELP was obtained by the highly efficient, cost-effective, inverse transition cycling (ICT) method and tested in mice. Furthermore, safety and immunogenicity of the crude tobacco leaf extracts were assessed in piglets. Antibodies recognizing mycobacterial antigens were produced in mice and piglets. A T-cell immune response able to recognize the native mycobacterial antigens was detected in mice. These findings showed that the native Ag85B and ESAT-6 mycobacterial B- and T-cell epitopes were conserved in the plant-expressed TBAg-ELP. This study presents the first results of an efficient plant-expression system, relying on the elastin-like peptide fusion strategy, to produce a safe and immunogenic mycobacterial Ag85B-ESAT-6 fusion protein as a potential vaccine candidate against tuberculosis.

Mots-clés Agrovoc : plante transgénique, tuberculose, vaccin, extrait d'origine végétale, Nicotiana tabacum, plante médicinale, bovin, genre humain, antigène, production végétale, Mycobacterium, immunogénétique, expression des gènes, fusion de gènes, génie génétique

Classification Agris : F30 - Génétique et amélioration des plantes
F60 - Physiologie et biochimie végétale
S50 - Santé humaine

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

• Floss Doreen Manuela, Leibniz Institute of Plant Genetics and Crop Plant Research (DEU)
• Mockey Michael
• Zanello Galliano, INRA (FRA)
• Brosson Damien, CNRS (FRA)
• Diogon Marie, CNRS (FRA)
• Frutos Roger, CIRAD-BIOS-UMR TRYPANOSOMES (FRA)
• Bruel Timothée, INRA (FRA)
• Rodrigues Valérie, CIRAD-BIOS-UMR CMAEE (FRA)
• Garzon Edwin
• Chevaleyre Claire, INRA (FRA)
• Berri Mustapha, INRA (FRA)
• Salmon Henri, INRA (FRA)
• Conrad Udo, Leibniz Institute of Plant Genetics and Crop Plant Research (DEU)
• Dedieu Laurence, CIRAD-BIOS-DIR (FRA) ORCID: 0000-0002-6492-3002

Source : Cirad - Agritrop (https://agritrop.cirad.fr/556401/)

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