Application of PCR-DGGE (Polymerase chain reaction denaturing) to analyze microbial ecosystems in foods

Brabet Catherine, Montet Didier, El Sheikha Aly, Le Nguyen Doan Duy. 2009. Application of PCR-DGGE (Polymerase chain reaction denaturing) to analyze microbial ecosystems in foods. In : International Society for Mycotoxicology (ISM) " Worldwide mycotoxin reduction in food and feed chains", 9-11 september 2009, Vienna, Austria. s.l. : s.n., Résumé, 1 p. International Society for Mycotoxicology (ISM) Conference, Vienne, Autriche, 9 Septembre 2009/11 Septembre 2009.

Résumé : The determination of geographical origin is a requirement of traceability system of import and export of food products. To this end, molecular techniques using 16S, 26S rDNA profiles generated by PCR-DGGE were used to detect the variation of the microbial community (bacteria, yeasts) structures of Pangasius fish from Viet Nam harvested in different aquaculture farms and during different seasons and two fruits from Physalis. If Physalis, the ecology of the yeast was also studied in the same purpose. Bacterial band profiles and mandarin fish, yeast and the Gaza Physalis specific profiles for each location and can be used as a bar code to certify the origin of fish and fruit. This method is a new tracking tool, which provides organic food with a unique bar code and an audit trail of food to their original location.

Classification Agris : U30 - Méthodes de recherche
Q04 - Composition des produits alimentaires
E70 - Commerce, commercialisation et distribution

Auteurs et affiliations

• Brabet Catherine, CIRAD-PERSYST-UMR Qualisud (FRA)
• Montet Didier, CIRAD-PERSYST-UMR Qualisud (FRA)
• El Sheikha Aly, CIRAD-PERSYST-UMR Qualisud (FRA)
• Le Nguyen Doan Duy, Can Tho University (VNM)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/559548/)

Métriques

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