Effects of organic and inorganic fertilization on soil bacterial and fungal microbial diversity in the Kabete long-term trial, Kenya

Kamaa Mary M., Mburu Harrison N., Blanchart Eric, Chibole Livingstone, Chotte Jean-Luc, Kibunja Catherine N., Lesueur Didier. 2011. Effects of organic and inorganic fertilization on soil bacterial and fungal microbial diversity in the Kabete long-term trial, Kenya. Biology and Fertility of Soils, 47 (3) : 315-321.

https://doi.org/10.1007/s00374-011-0539-3

Article de revue ; Article de revue à facteur d'impact

Version publiée - Anglais
Accès réservé aux personnels Cirad
Utilisation soumise à autorisation de l'auteur ou du Cirad.
document_559714.pdf
Télécharger (259kB)

Quartile : Q1, Sujet : SOIL SCIENCE

Résumé : The effects of crop manure and inorganic fertilizers on composition of microbial communities of central high land soils of Kenya are poorly known. For this reason, we have carried out a thirty-two-year-old long-term trial in Kabete, Kenya. These soils were treated with organic (maize stover (MS) at 10 t ha?1, farmyard manure (FYM) at 10 t ha?1) and inorganic fertilizers 120 kg N, 52.8 kg P (N2P2), N2P2+MS, N2P2+FYM, a control, and a fallow for over 30 years. We examined 16S rRNA gene and 28S rRNA gene fingerprints of bacterial and fungal diversity by PCR amplification and denaturing gradient gel electrophoresis separation, respectively. The PCR bacterial community structure and diversity were negatively affected by N2P2 and were more closely related to the bacterial structure in the soils without any addition (control) than that of soils with a combination of inorganic and organic or inorganic fertilizers alone. The effect on fungal diversity by N2P2 was different than the effect on bacterial diversity since the fungal diversity was similar to that of the N2P2+FYM and N2P2+MS-treated. However, soils treated with organic inputs clustered away from soils amended with inorganic inputs. Organic inputs had a positive effect on both bacterial and fungal diversity with or without chemical fertilizers. Results from this study suggested that total diversity of bacterial and fungal communities was closely related to agro-ecosystem management practices and may partially explain the yield differences observed between the different treatments.

Mots-clés Agrovoc : fertilisation, fertilité du sol, engrais organique, engrais minéral, micro-organisme du sol, flore microbienne, flore du sol, agroécosystème, biodiversité, génétique des populations, PCR, pratique culturale, rendement des cultures