Isolation and characterization of microsatellite markers of an endangered tropical tree, Sideroxylon majus, and cross-species amplification in other Sapotaceae species

Dafreville Stéphanie, Payet Guillaume, Simiand Christophe, Risterucci Ange-Marie, Riviere Expédit, Lebreton Gérard, Humeau Laurence, Strasberg Dominique, Chevallier Marie-Hélène. 2011. Isolation and characterization of microsatellite markers of an endangered tropical tree, Sideroxylon majus, and cross-species amplification in other Sapotaceae species. Conservation Genetics Resources, 3 (4) : 701-704.

https://doi.org/10.1007/s12686-011-9437-0

Article de revue ; Article de revue à facteur d'impact

Version publiée - Anglais
Accès réservé aux personnels Cirad
Utilisation soumise à autorisation de l'auteur ou du Cirad.
document_561334.pdf
Télécharger (153kB)

Quartile : Q4, Sujet : BIODIVERSITY CONSERVATION / Quartile : Q4, Sujet : GENETICS & HEREDITY

Résumé : Sideroxylon majus (Sapotaceae) is an endangered endemic tree of La Re´union Island that has suffered from human actions. It is present in small and isolated populations that encounter severe difficulties to regenerate. To have powerful tools for population genetic studies, we have isolated and characterized 14 polymorphic microsatellite markers from S. majus. The 14 loci were tested on 57 individuals from 6 populations. The number of alleles per locus varied from 2 to 20, with an average of 11.8. The observed and expected heterozygosity levels ranged from 0.053 to 1.000, and 0.116 to 0.917, respectively. Six of the 14 loci deviated from Hardy-Weinberg equilibrium. These polymorphic microsatellite markers constitute new tools to study the genetic diversity and spatial genetic structure of S. majus. The cross-species amplifications indicate that most of these loci can be used to investigate population genetic structure in S. grandiflorum, S. boutonianum and S. sessiliflorum. These studies will provide useful results for the elaboration of effective conservation strategies.

Mots-clés Agrovoc : Sapotaceae, espèce en danger, marqueur génétique, microsatellite, identification, conservation des ressources génétiques, génie génétique

Mots-clés géographiques Agrovoc : La Réunion, France

Mots-clés complémentaires : Sideroxylon majus

Classification Agris : F30 - Génétique et amélioration des plantes
F70 - Taxonomie végétale et phytogéographie
P01 - Conservation de la nature et ressources foncières

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations