Optimization of pathogenicity assays to study the Arabidopsis thaliana-Xanthomonas campestris pv. campastris pathosystem

Meyer Damien, Lauber Emmanuelle, Roby Dominique, Arlat Mathieu, Kroj Thomas. 2005. Optimization of pathogenicity assays to study the Arabidopsis thaliana-Xanthomonas campestris pv. campastris pathosystem. Molecular Plant Pathology, 6 (3) : 327-333.

Résumé : The cruciferous weed Arabidopsis thaliana and the causal agent of black rot disease of Crucifers Xanthomonas campestris pv. campestris ( Xcc ) are both model organisms in plant pathology. Their interaction has been studied successfully in the past, but these investigations suffered from high variability. In the present study, we describe an improved Arabidopsis- Xcc pathosystem that is based on a wound inoculation procedure. We show that after wound inoculation, Xcc colonizes the vascular system of Arabidopsis leaves and causes typical black rot symptoms in a compatible interaction, while in an incompatible interaction bacterial multiplication is inhibited. The highly synchronous and reproducible symptom expression allowed the development of a disease scoring scheme that enabled us to analyse the effects of mutations in individual genes on plant resistance or on bacterial virulence in a simple and precise manner. This optimized Arabidopsis- Xcc pathosystem will be a robust tool for further genetic and post-genomic investigation of fundamental questions in plant pathology.

Mots-clés Agrovoc : Arabidopsis thaliana, Xanthomonas campestris, pouvoir pathogène, pathologie végétale, relation hôte pathogène, résistance génétique, mutation provoquée, facteur du milieu

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Meyer Damien, INRA (FRA) ORCID: 0000-0003-2735-176X
• Lauber Emmanuelle, INRA (FRA)
• Roby Dominique, INRA (FRA)
• Arlat Mathieu, INRA (FRA)
• Kroj Thomas, INRA (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/561842/)

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