RNA interference against animal viruses : How morbilliviruses generate extended diversity to escape small interfering RNA control

Holz Carine, Albina Emmanuel, Minet Cécile, Lancelot Renaud, Kwiatek Olivier, Libeau Geneviève, Servan de Almeida Renata. 2012. RNA interference against animal viruses : How morbilliviruses generate extended diversity to escape small interfering RNA control. Journal of Virology, 86 (2) : 786-795.

https://doi.org/10.1128/JVI.06210-11

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Quartile : Q1, Sujet : VIROLOGY

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Résumé : Viruses are serious threats to human and animal health. Vaccines can prevent viral diseases, but few antiviral treatments are available to control evolving infections. Among new antiviral therapies, RNA interference (RNAi) has been the focus of intensive research. However, along with the development of efficient RNAi-based therapeutics comes the risk of emergence of resistant viruses. In this study, we challenged the in vitro propensity of a morbillivirus (peste des petits ruminants virus), a stable RNA virus, to escape the inhibition conferred by single or multiple small interfering RNAs (siRNAs) against conserved regions of the N gene. Except with the combination of three different siRNAs, the virus systematically escaped RNAi after 3 to 20 consecutive passages. The genetic modifications involved consisted of single or multiple point nucleotide mutations and a deletion of a stretch of six nucleotides, illustrating that this virus has an unusual genomic malleability.

Mots-clés Agrovoc : morbillivirus, virus peste petits ruminants, contrôle de maladies, transcription, arn messager, variation génétique

Classification Agris : L73 - Maladies des animaux

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

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