Identification, characterization and mapping of EST-derived SSRs from the cacao-Ceratocystis cacaofunesta interaction

Ferreira Santos Rogério Mercês, Clément Didier, Santos Lima Livia, Legravre Thierry, Lanaud Claire, Schnell Raymond J., Pires José Luis, Lopes Uilson Vanderlei, Micheli Fabienne, Gramacho Karina Peres. 2013. Identification, characterization and mapping of EST-derived SSRs from the cacao-Ceratocystis cacaofunesta interaction. Tree Genetics and Genomes, 9 (1) : 117-127.

https://doi.org/10.1007/s11295-012-0539-y

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Quartile : Q1, Sujet : FORESTRY / Quartile : Q1, Sujet : HORTICULTURE / Quartile : Q3, Sujet : GENETICS & HEREDITY

Résumé : Ceratocystis cacaofunesta is an ascomycete responsible for the lethal wilt disease of cacao (Theobroma cacao L.). Marker-assisted selection combined with conventional breeding is one powerful approach to improve cacao resistance to Ceratocystis wilt. In this study we screened a set of ESTs obtained from cacao elicited with C. cacaofunesta to identify EST-SSRs and test their efficacy for mapping. Among the 3,432 ESTs analysed, 384 contained SSRs and 428 EST-SSRs were identified, mainly dinucleotides (78.5 %), with four repeats (75.23 %), and preferentially AG/CT motif (25.47 %). Gene ontology function was assigned to the ESTs containing SSRs: 4.04 % belonged to "defense response" category, with 20.69 % of them to the sub-category "defense response to fungi". In relation to the ORF, the same quantity of EST-SSRs was observed in the 5? UTR, ORF and the 3? UTR (about 30 %). From the 428 EST-SSRs identified, 12 were polymorphic, revealing a total of 41 alleles. The number of alleles per locus ranged from 2 to 6, with an average of 3.41. Four EST-SSRs were mapped on the F2 Sca 6 × ICS 1 population segregating for Ceratocystis wilt, and were distributed on the 2, 3, 4 and 8 linkage groups. These markers will have potential applications in linkage mapping and will be valuable for the research community to improve the cacao breeding program.

Mots-clés Agrovoc : Ceratocystis, Theobroma cacao, identification, carte génétique, polymorphisme génétique, marqueur génétique, résistance aux maladies, résistance génétique, génétique moléculaire

Mots-clés géographiques Agrovoc : Brésil, Venezuela (République bolivarienne du), Équateur, Guyane française, Pérou, Colombie, Trinité-et-Tobago, France

Mots-clés complémentaires : SSR, Ceratocystis cacaofunesta

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

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