Beauveria brongniartii fungus infecting white grubs attacking sugarcane in the Kwazulu-Natal midlands north region

Goble Tarryn, Costet Laurent, Robène Isabelle, Nibouche Samuel, Rutherford R.S., Conlong Desmond, Hill M.P.. 2012. Beauveria brongniartii fungus infecting white grubs attacking sugarcane in the Kwazulu-Natal midlands north region. Proceedings of the Annual Congress of the South African Sugar Technologists' Association, 85 : 118-122. Annual Congress of the South African Sugar Technologists' Association (SASTA). 85, Durban, Afrique du Sud, 15 Août 2012/17 Août 2012.

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Résumé : Beauveria brongniartii (Saccardo) Petch epizootics were recorded at two sites in the Dalton area of the Midlands in South Africa on the melolonthid species, Hypopholis sommeri Burmeister (Coleoptera: Scarabaeidae). To identify the disease-causing fungus, 17 different fluorescently-labelled microsatellite PCR primers were used to target 78 isolates of Beauveria spp. DNA. Microsatellite data resolved two distinct clusters of Beauveria isolates which represented the B. bassiana s.s. (Balsamo) Vuillemin and B. brongniartii species groups. Groupings were supported by two gene regions, the nuclear ribosomal ITS and Bloc, of which 38 exemplar Beauveria isolates were represented and sequenced. Microsatellite analyses also showed that B. brongniartii conidia were being cycled from epigeal to subterranean habitats and vice versa in the environment by H. sommeri beetles. This is the first record of this species of fungus causing epizootics on melolonthid larvae and adults of H. sommeri in South Africa.

Mots-clés Agrovoc : Saccharum officinarum, Beauveria, lutte biologique, variation génétique, phylogénie, microsatellite, PCR, Beauveria bassiana, identification

Mots-clés géographiques Agrovoc : Afrique du Sud

Mots-clés complémentaires : Hypopholis sommeri, Beauveria brongniartii

Classification Agris : H10 - Ravageurs des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

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