Isolation and characterization of six polymorphic microsatellite loci for the Malagasy spider tortoise, Pyxis arachnoides and cross-amplification in Pyxis planicauda

Orozco-terWengel Pablo, Chiari Ylenia, Vieites David R., Pedrono Miguel, Louis Jr Edward. 2013. Isolation and characterization of six polymorphic microsatellite loci for the Malagasy spider tortoise, Pyxis arachnoides and cross-amplification in Pyxis planicauda. Amphibia-Reptilia, 34 (1) : 125-128.

Quartile : Q2, Sujet : ZOOLOGY

Résumé : Pyxis arachnoides is a Critically Endangered Malagasy tortoise for which little data about its natural history and genetic variation are available. This lack of knowledge complicates undertaking conservation measures considering Pyxis genetic diversity. Here, we describe six new microsatellites for Pyxis arachnoides from wild specimens and several localities, and the cross amplification of two Astrochelys radiata microsatellites. Of these microsatellites, two are monomorphic among the three distinct subspecies and a closely related species, while the remaining six are polymorphic. Microsatellite data are needed for this species to study the inter- and intra-subspecies genetic diversity, especially for the highly endangered subspecies P. a. brygooi, which currently occurs only in remote localities. The availability of polymorphic microsatellites will allow identifying the population of origin of individuals and will aid in justifying their management as separate evolutionary lineages for conservation purposes. The rationale is to preserve both evolutionary processes and adaptive potential.

Mots-clés Agrovoc : Tortue terrestre, identification, microsatellite, variation génétique, polymorphisme génétique, génétique des populations, espèce en danger, animal sauvage

Mots-clés géographiques Agrovoc : Madagascar

Mots-clés complémentaires : Pyxis arachnoides, Pyxis planicauda, Astrochelys radiata

Classification Agris : L60 - Taxonomie et géographie animales
L10 - Génétique et amélioration des animaux

Champ stratégique Cirad : Hors axes (2005-2013)

Auteurs et affiliations

• Orozco-terWengel Pablo, University of Wales College of Cardiff (GBR)
• Chiari Ylenia, CIBIO (PRT)
• Vieites David R., CIBIO (PRT)
• Pedrono Miguel, CIRAD-ES-UPR AGIRs (MDG)
• Louis Jr Edward, Omaha's Henry Doorly Zoo and Aquarium's Center for Conservation and Research (USA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/567799/)

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