QTL mapping of growth-related traits in a full-sib family of rubber tree (Hevea brasiliensis) evaluated in a sub-tropical climate

Souza Livia Moura, Gazaffi Rodrigo, Mantello Camilia Campos, Silva Carla Cristina, Garcia Dominique, Le Guen Vincent, Cardoso Saulo Emilio Almeida, Garcia Antonio Augusto Franco, Souza Anete Pereira. 2013. QTL mapping of growth-related traits in a full-sib family of rubber tree (Hevea brasiliensis) evaluated in a sub-tropical climate. PloS One, 8 (4):e61238, 10 p.

https://doi.org/10.1371/journal.pone.0061238

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Quartile : Outlier, Sujet : MULTIDISCIPLINARY SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Résumé : The rubber tree (Hevea spp.), cultivated in equatorial and tropical countries, is the primary plant used in natural rubber production. Due to genetic and physiological constraints, inbred lines of this species are not available. Therefore, alternative approaches are required for the characterization of this species, such as the genetic mapping of full-sib crosses derived from outbred parents. In the present study, an integrated genetic map was obtained for a full-sib cross family with simple sequence repeats (SSRs) and expressed sequence tag (EST-SSR) markers, which can display different segregation patterns. To study the genetic architecture of the traits related to growth in two different conditions (winter and summer), quantitative trait loci (QTL) mapping was also performed using the integrated map. Traits evaluated were height and girth growth, and the statistical model was based in an extension of composite interval mapping. The obtained molecular genetic map has 284 markers distributed among 23 linkage groups with a total length of 2688.8 cM. A total of 18 QTLs for growth traits during the summer and winter seasons were detected. A comparison between the different seasons was also conducted. For height, QTLs detected during the summer season were different from the ones detected during winter season. This type of difference was also observed for girth. Integrated maps are important for genetics studies in outbred species because they represent more accurately the polymorphisms observed in the genitors. QTL mapping revealed several interesting findings, such as a dominance effect and unique segregation patterns that each QTL could exhibit, which were independent of the flanking markers. The QTLs identified in this study, especially those related to phenotypic variation associated with winter could help studies of marker-assisted selection that are particularly important when the objective of a breeding program is to obtain phenotypes that are adapted to sub-optimal regions.

Mots-clés Agrovoc : Hevea brasiliensis, carte génétique, séquence nucléotidique, locus des caractères quantitatifs, phénotype, variation saisonnière, taux de croissance, zone subtropicale

Mots-clés géographiques Agrovoc : Mato Grosso

Mots-clés complémentaires : SSR

Classification Agris : F30 - Génétique et amélioration des plantes
F62 - Physiologie végétale - Croissance et développement

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

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