Molecular epidemiology of Orientia tsutsugamushi in Cambodia and Central Vietnam reveals a broad region-wide genetic diversity

Duong Veasna, Thinh Thi Xuan May, Blassdell Kim, Le Viet Lo, Morvan Claire, Sreyrath Lay, Anukool Wichittra, Wongprompitak Patimaporn, Suputtamongkol Yupin, Laurent Denis, Richner Beat, Ra Chheang, Bui Trong Chien, Frutos Roger, Buchy Philippe. 2013. Molecular epidemiology of Orientia tsutsugamushi in Cambodia and Central Vietnam reveals a broad region-wide genetic diversity. Infection, Genetics and Evolution, 15, spec. : pp. 35-42. International Giardia and Cryptosporidium Conference. 4, Wellington, Afrique du Sud, 30 January 2012/3 February 2012.

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Quartile : Q2, Sujet : INFECTIOUS DISEASES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Anthropologie-Ethnologie

Abstract : Scrub typhus is an acute infectious disease caused by an obligate intracellular bacterium Orientia tsutsugamushi following the bite of infected trombiculid mites of the genus Leptotrombidium. This zoonotic disease is a major cause of febrile illness in the Asia-Pacific region, with a large spectrum of clinical manifestations from unapparent or mild disease to fatal disease. O. tsutsugamushi is characterized by a very high genomic plasticity and a large number of antigenic variants amongst strains. The 56-kDa type specific antigen (TSA) gene, encoding the major antigenic protein, was used as reference to investigate the genetic relationships between the strains and to genotype O. tsutsugamushi isolates. The open reading frame of the 56-kDa TSA gene of 41 sequences (28 Cambodian and 13 Vietnamese strains) from patient samples were sequenced and used for genotyping. The 28 Cambodian isolates clustered into 5 major groups, including Karp (43.5%), JG-v (25%), Kato/TA716 (21.5%), TA763 (3.5%) and Gilliam (3.5%). Karp (77%), TA763 (15.5%) and JG-v (7.5%) strains were identified amongst the 13 Vietnamese isolates. This is the first countrywide genotyping description in Cambodia and in Central Vietnam. These results demonstrate the considerable diversity of genotypes in co-circulation in both countries. The genotyping result might raise awareness amongst Cambodian and Vietnamese clinicians of the high genetic diversity of circulating O. tsutsugamushi strains and provides unique and beneficial data for serological and molecular diagnosis of scrub typhus infections as well as raw materials for future studies and vaccine development. (Résumé d'auteur)

Mots-clés Agrovoc : Rickettsiales, Phylogénie, Variation génétique, Génotype, Antigène, Leptotrombidium, Diagnostic, Genre humain

Mots-clés géographiques Agrovoc : Cambodge, Viet Nam

Mots-clés complémentaires : Orientia tsutsugamushi

Classification Agris : L73 - Animal diseases
000 - Other themes

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

  • Duong Veasna, Institut Pasteur du Cambodge (KHM)
  • Thinh Thi Xuan May, Institut Pasteur de Ngatrang (VNM)
  • Blassdell Kim, Institut Pasteur du Cambodge (KHM)
  • Le Viet Lo, Institut Pasteur de Ngatrang (VNM)
  • Morvan Claire, Institut Pasteur du Cambodge (KHM)
  • Sreyrath Lay, Institut Pasteur du Cambodge (KHM)
  • Anukool Wichittra, Mahidol University (THA)
  • Wongprompitak Patimaporn, Mahidol University (THA)
  • Suputtamongkol Yupin, Mahidol University (THA)
  • Laurent Denis, Kantha Bopha Hospital (KHM)
  • Richner Beat, Kantha Bopha Hospital (KHM)
  • Ra Chheang, Calmette Hospital (KHM)
  • Bui Trong Chien, Institut Pasteur de Ngatrang (VNM)
  • Buchy Philippe, Institut Pasteur du Cambodge (KHM)

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