Salt-responsive ERF1 regulates reactive oxygen species-dependent signaling during the initial response to salt stress in rice

Schmidt Romy, Mieulet Delphine, Hubberten Hans-Michael, Obata Toshihiro, Hoefgen Rainer, Fernie Alisdair R., Fisahn Joachim, San Segundo Blanca, Guiderdoni Emmanuel, Schippers Jos H.M., Mueller-Roeber Bernd. 2013. Salt-responsive ERF1 regulates reactive oxygen species-dependent signaling during the initial response to salt stress in rice. Plant Cell, 25 (6) : 2115-2131.

Quartile : Outlier, Sujet : PLANT SCIENCES / Quartile : Outlier, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY / Quartile : Q1, Sujet : CELL BIOLOGY

Résumé : Early detection of salt stress is vital for plant survival and growth. Still, the molecular processes controlling early salt stress perception and signaling are not fully understood. Here, we identified SALT-RESPONSIVE ERF1 (SERF1), a rice (Oryza sativa) transcription factor (TF) gene that shows a root-specific induction upon salt and hydrogen peroxide (H2O2) treatment. Loss of SERF1 impairs the salt-inducible expression of genes encoding members of a mitogen-activated protein kinase (MAPK) cascade and salt tolerance-mediating TFs. Furthermore, we show that SERF1-dependent genes are H2O2 responsive and demonstrate that SERF1 binds to the promoters of MAPK KINASE KINASE6 (MAP3K6), MAPK5, DEHYDRATION-RESPONSIVE ELEMENT BINDING2A (DREB2A), and ZINC FINGER PROTEIN179 (ZFP179) in vitro and in vivo. SERF1 also directly induces its own gene expression. In addition, SERF1 is a phosphorylation target of MAPK5, resulting in enhanced transcriptional activity of SERF1 toward its direct target genes. In agreement, plants deficient for SERF1 are more sensitive to salt stress compared with the wild type, while constitutive overexpression of SERF1 improves salinity tolerance. We propose that SERF1 amplifies the reactive oxygen species-activated MAPK cascade signal during the initial phase of salt stress and translates the salt-induced signal into an appropriate expressional response resulting in salt tolerance.

Mots-clés Agrovoc : Oryza sativa, tolérance au sel, stress osmotique, salinité, péroxyde, résistance génétique, expression des gènes, plante transgénique, transformation génétique, sel, dérivé réactif de l'oxygène

Mots-clés complémentaires : Peroxyde d'oxygène

Classification Agris : H50 - Troubles divers des plantes
F60 - Physiologie et biochimie végétale
F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Schmidt Romy, University of Potsdam (DEU)
• Mieulet Delphine, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-6220-0372
• Hubberten Hans-Michael, Max Planck Institute of Molecular Plant Physiology (DEU)
• Obata Toshihiro, Max Planck Institute of Molecular Plant Physiology (DEU)
• Hoefgen Rainer, Max Planck Institute of Molecular Plant Physiology (DEU)
• Fernie Alisdair R., Max Planck Institute of Molecular Plant Physiology (DEU)
• Fisahn Joachim, Max Planck Institute of Molecular Plant Physiology (DEU)
• San Segundo Blanca, Max Planck Institute of Molecular Plant Physiology (DEU)
• Guiderdoni Emmanuel, CIRAD-BIOS-UMR AGAP (FRA)
• Schippers Jos H.M., University of Potsdam (DEU)
• Mueller-Roeber Bernd, University of Potsdam (DEU)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/570949/)

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