Investigating natural rubber composition with Fourier Transform Infrared (FT-IR) spectroscopy: A rapid and non-destructive method to determine both protein and lipid contents simultaneously

Rolere Sébastien, Liengprayoon Siriluck, Vaysse Laurent, Sainte-Beuve Jérôme, Bonfils Frédéric. 2015. Investigating natural rubber composition with Fourier Transform Infrared (FT-IR) spectroscopy: A rapid and non-destructive method to determine both protein and lipid contents simultaneously. Polymer Testing, 43 : 83-93.

https://doi.org/10.1016/j.polymertesting.2015.02.011

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Quartile : Q1, Sujet : MATERIALS SCIENCE, CHARACTERIZATION & TESTING / Quartile : Q2, Sujet : POLYMER SCIENCE

Résumé : A large panel of Natural Rubber (NR) samples was characterized using Fourier Transform Infrared (FT-IR) spectroscopy in Attenuated Total Reflection (ATR) configuration. Specific vibrational bands were attributed to some non-isoprene compounds naturally present in NR composition. A rapid and non-destructive method was developed to investigate some specific functional groups contained in lipids (ester and carboxyl groups) and proteins (amides). Ester and carboxyl groups were quantified using calibration curves developed from synthetic cis-1,4-polyisoprene mixtures with either methyl stearate or stearic acid. Amide groups of proteins and peptides were found to be directly quantifiable from NR FTIR spectra. The clonal origin and processing were found to influence the non-isoprene composition of NR. Significant correlations were found between FT-IR results and conventional chemical analyses: nitrogen content for proteins and total lipid extract.

Mots-clés Agrovoc : caoutchouc, Hevea brasiliensis, spectroscopie infrarouge, clone, teneur en protéines, teneur en lipides, testage non destructif

Mots-clés géographiques Agrovoc : Côte d'Ivoire, Thaïlande, Indonésie, Viet Nam

Classification Agris : Q60 - Traitement des produits agricoles non alimentaires
U30 - Méthodes de recherche
F60 - Physiologie et biochimie végétale

Champ stratégique Cirad : Axe 2 (2014-2018) - Valorisation de la biomasse

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