Ethylene response factors are controlled by multiple harvesting stresses in Hevea brasiliensis

Putranto Riza Arief, Duan Cuifang, Kuswanhadi, Chaidamsari Tetty, Rio Maryannick, Piyatrakul Piyanuch, Herlinawati Eva, Pirrello Julien, Dessailly Florence, Leclercq Julie, Bonnot François, Tang Chaorong, Hu Songnian, Montoro Pascal. 2015. Ethylene response factors are controlled by multiple harvesting stresses in Hevea brasiliensis. PloS One, 10 (4):e0123618, 26 p.

https://doi.org/10.1371/journal.pone.0123618

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Quartile : Q1, Sujet : MULTIDISCIPLINARY SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Résumé : Tolerance of recurrent mechanical wounding and exogenous ethylene is a feature of the rubber tree. Latex harvesting involves tapping of the tree bark and ethephon is applied to increase latex flow. Ethylene is an essential element in controlling latex production. The ethylene signalling pathway leads to the activation of Ethylene Response Factor (ERF) transcription factors. This family has been identified in Hevea brasiliensis. This study set out to understand the regulation of ERF genes during latex harvesting in relation to abiotic stress and hormonal treatments. Analyses of the relative transcript abundance were carried out for 35 HbERF genes in latex, in bark from mature trees and in leaves from juvenile plants under multiple abiotic stresses. Twenty-one HbERF genes were regulated by harvesting stress in laticifers, revealing an overrepresentation of genes in group IX. Transcripts of three HbERF-IX genes from HbERF-IXc4, HbERF-IXc5 and HbERF-IXc6 were dramatically accumulated by combining wounding, methyl jasmonate and ethylene treatments. When an ethylene inhibitor was used, the transcript accumulation for these three genes was halted, showing ethylene-dependent induction. Subcellular localization and transactivation experiments confirmed that several members of HbERF-IX are activator-type transcription factors. This study suggested that latex harvesting induces mechanisms developed for the response to abiotic stress. These mechanisms probably depend on various hormonal signalling pathways. Severalmembers of HbERF-IX could be essential integrators of complex hormonal signalling pathways in Hevea.

Mots-clés Agrovoc : Hevea brasiliensis, latex, stress abiotique, expression des gènes, réponse de la plante, éthylène, Transcription génique, métabolisme, régulation physiologique, éthéphon, substance de croissance végétale, stade de développement végétal