Maximum-likelihood method identifies meiotic restitution mechanism from heterozygosity transmission of centromeric loci: Application in citrus

Cuenca José, Aleza Pablo, Juarez José, Garcia-Lor Andrés, Froelicher Yann, Navarro Luis, Ollitrault Patrick. 2015. Maximum-likelihood method identifies meiotic restitution mechanism from heterozygosity transmission of centromeric loci: Application in citrus. Scientific Reports, 5 (9897), 11 p.

Journal article ; Article de revue à facteur d'impact Revue en libre accès total
Published version - Anglais
Use under authorization by the author or CIRAD.

Télécharger (1MB) | Preview


Abstract : Polyploidisation is a key source of diversification and speciation in plants. Most researchers consider sexual polyploidisation leading to unreduced gamete as its main origin. Unreduced gametes are useful in several crop breeding schemes. Their formation mechanism, i.e., First-Division Restitution (FDR) or Second-Division Restitution (SDR), greatly impacts the gametic and population structures and, therefore, the breeding efficiency. Previous methods to identify the underlying mechanism required the analysis of a large set of markers over large progeny. This work develops a new maximum-likelihood method to identify the unreduced gamete formation mechanism both at the population and individual levels using independent centromeric markers. Knowledge of marker-centromere distances greatly improves the statistical power of the comparison between the SDR and FDR hypotheses. Simulating data demonstrated the importance of selecting markers very close to the centromere to obtain significant conclusions at individual level. This new method was used to identify the meiotic restitution mechanism in nineteen mandarin genotypes used as female parents in triploid citrus breeding. SDR was identified for 85.3% of 543 triploid hybrids and FDR for 0.6%. No significant conclusions were obtained for 14.1% of the hybrids. At population level SDR was the predominant mechanisms for the 19 parental mandarins. (Résumé d'auteur)

Mots-clés Agrovoc : Citrus, Locus, Gamétogénèse, Technique analytique, Hétérozygote, Méiose, Polyploïdie, Physiologie de la reproduction, Reproduction sexuée, Cytogénétique, Marqueur génétique, Distance génétique, Modèle mathématique

Classification Agris : F30 - Plant genetics and breeding
F63 - Plant physiology - Reproduction

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Cuenca José, IVIA (ESP)
  • Aleza Pablo, IVIA (ESP)
  • Juarez José, IVIA (ESP)
  • Garcia-Lor Andrés, IVIA (ESP)
  • Froelicher Yann, CIRAD-BIOS-UMR AGAP (FRA)
  • Navarro Luis, IVIA (ESP)
  • Ollitrault Patrick, CIRAD-BIOS-UMR AGAP (ESP) ORCID: 0000-0002-9456-5517

Source : Cirad-Agritrop (

View Item (staff only) View Item (staff only)

[ Page générée et mise en cache le 2021-02-27 ]