Resistance to normal human serum reveals Trypanosoma lewisi as an underestimated human pathogen

Lun Zhao-Rong, Wen Yan-Zi, Uzureau Pierrick, Lecordier Laurence, Lai De-Hua, Lan You-Gen, Desquesnes Marc, Gengler Nicolas, Yang Ting-Bao, Zhou Wen-Liang, Jannin Jean, Simarro Pere P., Truc Philippe, Vincendeau Philippe, Pays Olivier. 2015. Resistance to normal human serum reveals Trypanosoma lewisi as an underestimated human pathogen. Molecular and Biochemical Parasitology, 199 (1-2) : 58-61.

https://doi.org/10.1016/j.molbiopara.2015.03.007

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Quartile : Q2, Sujet : PARASITOLOGY / Quartile : Q3, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Résumé : Human-infectious trypanosomes such as Trypanosoma cruzi, T. brucei rhodesiense, and T. b. gambiense can be discriminated from those only infecting animals by their resistance to normal human serum (NHS). These parasites are naturally resistant to trypanolysis induced by the human-specific pore-forming serum protein apolipoprotein L1 (ApoL-1). T. lewisi, a worldwide distributed parasite, has been considered as rat-specific and non-pathogenic to the natural hosts. Here we provide evidence that 19 tested T. lewisi isolates from Thailand and China share resistance to NHS. Further investigation on one selected isolate CPO02 showed that it could resist at least 90% NHS or 30 μg/ml recombinant human ApoL-1 (rhApoL-1) in vitro, in contrast to T. b. brucei which could not survive in 0.0001% NHS and 0.1 μg/ml rhApoL-1. In vivo tests in rats also demonstrated that this parasite is fully resistant to lysis by NHS. Together with recent reports of atypical human infection by T. lewisi, these data allow the conclusion that T. lewisi is potentially an underestimated and thus a neglected human pathogen.

Mots-clés Agrovoc : Trypanosoma lewisi, agent pathogène, maladie de l'homme, genre humain, résistance aux maladies, apoprotéine

Mots-clés géographiques Agrovoc : Chine, Thaïlande

Mots-clés complémentaires : Sérum

Classification Agris : L73 - Maladies des animaux
000 - Autres thèmes
L72 - Organismes nuisibles des animaux

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

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