Isolation of Bartonella henselae and two new Bartonella subspecies, Bartonella koehlerae Subspecies boulouisii subsp. nov. and Bartonella koehlerae Subspecies bothieri subsp. nov. from free-ranging Californian mountain lions and bobcats

Chomel Bruno B., Molia Sophie, Kasten Rickie W., Borgo Gina M., Stuckey Matthew J., Maruyama Soichi, Chang Chao-chin, Haddad Nadia, Koehler Jane E.. 2016. Isolation of Bartonella henselae and two new Bartonella subspecies, Bartonella koehlerae Subspecies boulouisii subsp. nov. and Bartonella koehlerae Subspecies bothieri subsp. nov. from free-ranging Californian mountain lions and bobcats. PloS One, 11 (3):e0148299, 21 p.

https://doi.org/10.1371/journal.pone.0148299

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Url - jeu de données - Entrepôt autre : http://www.ncbi.nlm.nih.gov/nuccore/KF246530

Quartile : Q1, Sujet : MULTIDISCIPLINARY SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Note générale : Jeux de données : All relevant data are within the paper and have been submitted to GenBank. The GenBank/EMBL/DDBJ accession numbers for the partial sequences of the gltA, ftsZ, rpoB genes and ITS regions of the two reference strains described in this paper are as follows: Bartonella koehlerae subsp. boulouisii (L-42-94), KF246521, KF246530, KF246539, and KF437493, respectively, and for Bartonella koehlerae subsp. bothieri (L-17-96), KF246528, KF246537, KF246545, and KF437500, respectively. GenBank Accession numbers for partial sequences of the above four loci in the other isolates described in this paper are summarized in Table 1.

Résumé : Domestic cats are the natural reservoir of Bartonella henselae, B. clarridgeiae and B. koehlerae. To determine the role of wild felids in the epidemiology of Bartonella infections, blood was collected from 14 free-ranging California mountain lions (Puma concolor) and 19 bobcats (Lynx rufus). Bartonella spp. were isolated from four (29%) mountain lions and seven (37%) bobcats. These isolates were characterized using growth characteristics, biochemical reactions, molecular techniques, including PCR-RFLP of selected genes or interspacer region, pulsed-field gel electrophoresis (PFGE), partial sequencing of several genes, and DNA-DNA hybridization. Two isolates were identical to B. henselae genotype II. All other isolates were distinguished from B. henselae and B. koehlerae by PCR-RFLP of the gltA gene using endonucleases HhaI, TaqI and AciI, with the latter two discriminating between the mountain lion and the bobcat isolates. These two novel isolates displayed specific PFGE profiles distinct from B. henselae, B. koehlerae and B. clarridgeiae. Sequences of amplified gene fragments from the three mountain lion and six bobcat isolates were closely related to, but distinct from, B. henselae and B. koehlerae. Finally, DNA-DNA hybridization studies demonstrated that the mountain lion and bobcat strains are most closely related to B. koehlerae. We propose naming the mountain lion isolates B. koehlerae subsp. boulouisii subsp. nov. (type strain: L-42-94), and the bobcat isolates B. koehlerae subsp. bothieri subsp. nov. (type strain: L-17-96), and to emend B. koehlerae as B. koehlerae subsp. koehlerae. The mode of transmission and the zoonotic potential of these new Bartonella subspecies remain to be determined.

Mots-clés Agrovoc : Bartonella henselae, chat, Felidae, zoonose, transmission des maladies, bartonella, espèce, identification, taxonomie, animal domestique, animal sauvage, interactions biologiques, PCR, RFLP, biologie moléculaire, phylogénie, immunologie, espèce nouvelle