Cellular and pectin dynamics during abscission zone development and ripe fruit abscission of the monocot oil palm

Roongsattham Peerapat, Morcillo Fabienne, Fooyontphanich Kim, Jantasuriyarat Chatchawan, Tragoonrung Somvong, Amblard Philippe, Collin Myriam, Mouille Gregory, Verdeil Jean-Luc, Tranbarger Timothy John. 2016. Cellular and pectin dynamics during abscission zone development and ripe fruit abscission of the monocot oil palm. Frontiers in Plant Science, 7 (540), 15 p.

Quartile : Q1, Sujet : PLANT SCIENCES

Résumé : The oil palm (Elaeis guineensis Jacq.) fruit primary abscission zone (AZ) is a multi-cell layered boundary region between the pedicel (P) and mesocarp (M) tissues. To examine the cellular processes that occur during the development and function of the AZ cell layers, we employed multiple histological and immunohistochemical methods combined with confocal, electron and Fourier-transform infrared (FT-IR) microspectroscopy approaches. During early fruit development and differentiation of the AZ, the orientation of cell divisions in the AZ was periclinal compared with anticlinal divisions in the P and M. AZ cell wall width increased earlier during development suggesting cell wall assembly occurred more rapidly in the AZ than the adjacent P and M tissues. The developing fruit AZ contain numerous intra-AZ cell layer plasmodesmata (PD), but very few inter-AZ cell layer PD. In the AZ of ripening fruit, PD were less frequent, wider, and mainly intra-AZ cell layer localized. Furthermore, DAPI staining revealed nuclei are located adjacent to PD and are remarkably aligned within AZ layer cells, and remain aligned and intact after cell separation. The polarized accumulation of ribosomes, rough endoplasmic reticulum, mitochondria, and vesicles suggested active secretion at the tip of AZ cells occurred during development which may contribute to the striated cell wall patterns in the AZ cell layers. AZ cells accumulated intracellular pectin during development, which appear to be released and/or degraded during cell separation. The signal for the JIM5 epitope, that recognizes low methylesterified and un-methylesterified homogalacturonan (HG), increased in the AZ layer cell walls prior to separation and dramatically increased on the separated AZ cell surfaces. Finally, FT-IR microspectroscopy analysis indicated a decrease in methylesterified HG occurred in AZ cell walls during separation, which may partially explain an increase in the JIM5 epitope signal. The results obtained through a multi-imaging approach allow an integrated view of the dynamic developmental processes that occur in a multi-layered boundary AZ and provide evidence for distinct regulatory mechanisms that underlie oil palm fruit AZ development and function.

Mots-clés Agrovoc : Elaeis guineensis, fruit (botanique), abscission, développement biologique, physiologie végétale, pectine, paroi cellulaire, microscopie, Microscopie électronique, spectroscopie infrarouge, mûrissage, anatomie végétale

Mots-clés géographiques Agrovoc : Bénin, Thaïlande

Classification Agris : F60 - Physiologie et biochimie végétale
F50 - Anatomie et morphologie des plantes
U30 - Méthodes de recherche

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

• Roongsattham Peerapat, IRD (FRA)
• Morcillo Fabienne, CIRAD-BIOS-UMR DIADE (FRA)
• Fooyontphanich Kim, IRD (FRA)
• Jantasuriyarat Chatchawan, Kasetsart University (THA)
• Tragoonrung Somvong, BIOTEC [National Center for Genetic Engineering and Biotechnology] (THA)
• Amblard Philippe, PalmElit (FRA)
• Collin Myriam, IRD (FRA)
• Mouille Gregory, Institut Jean-Pierre Bourgin (FRA)
• Verdeil Jean-Luc, CIRAD-BIOS-UMR AGAP (FRA)
• Tranbarger Timothy John, IRD (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/580774/)

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